Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | 1 mM GSH decreases Km and increases Vmax estimates for arsenite and methylarsonous acid | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
M287T | in the absence of glutathione, the mutant shows decreased Vmax and Km for arsenite and increased Vmax and Km for methylarsonate compared to the wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | addition of 20 mM GSH sharply inhibits methylation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
methylarsonate | wild type enzyme, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C | Homo sapiens | |
0.0009 | - |
methylarsonate | mutant enzyme M287T, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C | Homo sapiens | |
0.0015 | - |
methylarsonate | wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C | Homo sapiens | |
0.0016 | - |
arsenite | mutant enzyme M287T, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0016 | - |
arsenite | wild type enzyme, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C | Homo sapiens | |
0.002 | - |
arsenite | mutant enzyme M287T, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C | Homo sapiens | |
0.003 | - |
methylarsonate | mutant enzyme M287T, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C | Homo sapiens | |
0.0041 | - |
arsenite | wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + arsenite | - |
Homo sapiens | S-adenosyl-L-homocysteine + methylarsonate + dimethylarsinous acid + dimethylarsinic acid | - |
? | |
S-adenosyl-L-methionine + methylarsonate | - |
Homo sapiens | S-adenosyl-L-homocysteine + dimethylarsinous acid + dimethylarsinic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arsenic (+3 oxidation state) methyltransferase | - |
Homo sapiens |
AS3MT | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme in the pathway for methylation of arsenicals | Homo sapiens |