Crystallization (Comment) | Organism |
---|---|
atomistic molecular dynamics study. The binding of the substrate contributes to the stabilization of the structure of the full complex. Conformational changes influence the orientation of the pyrrole rings in the substrate. Conformotional motions lead to more open conformation of enzyme active site to accommodate the substrate | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E114Q | increase in the root mean square deviations of the uroporphyrinogen III substrate in respect to the wild-type | Pseudomonas aeruginosa |
G189K | increase in activity compared to wild-type. The side chain of K189 makes electrostatic interactions with the propionate side chain of ring B with an average distance of 4.0 A. The backbone of K189 makes hydrogen bonds with the propionate side chain of ring B of uroporphyrinogen III | Pseudomonas aeruginosa |
G189N | increase in activity compared to wild-type. Both side chain and the backbone of N189 residue participates in hydrogen bonds with the propionate side chain of ring B of uroporphyrinogen III | Pseudomonas aeruginosa |
M186L | loss of hydrophobic interactions between the side chain of L186 and methyl group of SAM | Pseudomonas aeruginosa |
R111K | the side chain of residue R111 stabilizes the uroporphyrinogen III ring A acetate side chain by electrostatic interactions. Mutant R111K makes very unstable interactions with an average distance of 5 A between the acetate of ring A of uroporphyrinogen III | Pseudomonas aeruginosa |
R51D | residue R51 is mainly involved in stabilization of the propionate side chain of ring A by hydrophobic interactions with an average distance of 4.1 A. In the mutant R51K, the side chain of K51 makes stable electrostatic interactions with the acetate side chain of ring A, however there are no hydrophobic interactions | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P95417 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
NirE | - |
Pseudomonas aeruginosa |