Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells and Nicotiana benthamiana | Goniophlebium amoenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04774 | - |
caffeoyl-CoA | isoform CCoAOMT1, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.04895 | - |
quercetin | isoform CCoAOMT1, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.05114 | - |
caffeoyl-CoA | isoform CCoAOMT2, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.05465 | - |
eriodictyol | isoform CCoAOMT2, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.09159 | - |
quercetin | isoform CCoAOMT2, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.09636 | - |
eriodictyol | isoform CCoAOMT1, at pH 7.5 and 37°C | Goniophlebium amoenum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Goniophlebium amoenum | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Goniophlebium amoenum | A0A4Y5R9F4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA Sefinose column chromatography | Goniophlebium amoenum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00379 | - |
isoform CCoAOMT1, with 5-hydroxyconiferaldehyde as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.00664 | - |
isoform CCoAOMT2, with caffeoyl aldehyde as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.00804 | - |
isoform CCoAOMT2, with caffeoyl alcohol as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.01107 | - |
isoform CCoAOMT2, with 5-hydroxyconiferaldehyde as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.01195 | - |
isoform CCoAOMT2, with baicalein as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.01332 | - |
isoform CCoAOMT1, with caffeoyl aldehyde as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.01476 | - |
isoform CCoAOMT2, with scutellarein as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.018 | - |
isoform CCoAOMT1, with eriodictyol as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.02313 | - |
isoform CCoAOMT1, with scutellarein as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.02643 | - |
isoform CCoAOMT1, with baicalein as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.02719 | - |
isoform CCoAOMT2, with caffeoyl-CoA as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.03484 | - |
isoform CCoAOMT1, with caffeoyl-CoA as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.03742 | - |
isoform CCoAOMT2, with luteolin as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.04316 | - |
isoform CCoAOMT2, with quercetin as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.04848 | - |
isoform CCoAOMT2, with eriodictyol as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.05571 | - |
isoform CCoAOMT1, with luteolin as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
0.05677 | - |
isoform CCoAOMT1, with quercetin as substrate, at pH 7.5 and 37°C | Goniophlebium amoenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is not able to methylate caffeic acid, 5-hydroxyferulic acid, 5-hydroxyconiferyl alcohol, kaempferol and naringenin | Goniophlebium amoenum | ? | - |
- |
|
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | - |
Goniophlebium amoenum | ? | - |
? | |
S-adenosyl-L-methionine + baicalein | - |
Goniophlebium amoenum | ? | - |
? | |
S-adenosyl-L-methionine + caffeoyl alcohol | - |
Goniophlebium amoenum | ? | - |
? | |
S-adenosyl-L-methionine + caffeoyl aldehyde | - |
Goniophlebium amoenum | ? | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Goniophlebium amoenum | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + eriodictyol | - |
Goniophlebium amoenum | ? | - |
? | |
S-adenosyl-L-methionine + luteolin | - |
Goniophlebium amoenum | ? | - |
? | |
S-adenosyl-L-methionine + quercetin | - |
Goniophlebium amoenum | ? | - |
? | |
S-adenosyl-L-methionine + scutellarein | - |
Goniophlebium amoenum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28750, isoform CCoAOMT1, calculated from amino acid sequence | Goniophlebium amoenum |
? | x * 32280, isoform CCoAOMT2, calculated from amino acid sequence | Goniophlebium amoenum |
Synonyms | Comment | Organism |
---|---|---|
caffeoyl coenzyme A 3-O-methyltransferase | - |
Goniophlebium amoenum |
CCoAOMT1 | isoform | Goniophlebium amoenum |
CCoAOMT2 | isoform | Goniophlebium amoenum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
caffeoyl-CoA | isoform CCoAOMT1, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.04 | - |
caffeoyl-CoA | isoform CCoAOMT2, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.05 | - |
eriodictyol | isoform CCoAOMT1, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.05 | - |
quercetin | isoform CCoAOMT2, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.13 | - |
eriodictyol | isoform CCoAOMT2, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.14 | - |
quercetin | isoform CCoAOMT1, at pH 7.5 and 37°C | Goniophlebium amoenum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme contributes to lignin synthesis | Goniophlebium amoenum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5303 | - |
quercetin | isoform CCoAOMT2, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.5309 | - |
eriodictyol | isoform CCoAOMT1, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.7388 | - |
caffeoyl-CoA | isoform CCoAOMT2, at pH 7.5 and 37°C | Goniophlebium amoenum | |
0.8783 | - |
caffeoyl-CoA | isoform CCoAOMT1, at pH 7.5 and 37°C | Goniophlebium amoenum | |
2.4386 | - |
eriodictyol | isoform CCoAOMT2, at pH 7.5 and 37°C | Goniophlebium amoenum | |
2.7637 | - |
quercetin | isoform CCoAOMT1, at pH 7.5 and 37°C | Goniophlebium amoenum |