Cloned (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
NADPH | pH 8.9, 65°C | Thermococcus litoralis | |
0.54 | - |
polysulfide(n) | pH 8.9, 65°C | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | B2BSJ7 and B2BSJ6 and B2BSJ5 and B2BSK0 | B2BSJ7: alpha subunit, B2BSJ6: beta subunit, B2BSJ5: gamma subunit, B2BSK0: delta subunit | - |
Thermococcus litoralis DSM 5473 | B2BSJ7 and B2BSJ6 and B2BSJ5 and B2BSK0 | B2BSJ7: alpha subunit, B2BSJ6: beta subunit, B2BSJ5: gamma subunit, B2BSK0: delta subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
pH 8.9, 65°C, purified recombinant subunit NsoC, oxidation of NADPH in the presence of polysulfide | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polysulfide(n) + NADPH + H+ | NADH can not replace NADPH, the purified recombinant enzyme catalyzes the reduction of polysulfide with NADPH as an electron donor and it also reduces oxygen | Thermococcus litoralis | hydrogen sulfide + polysulfide(n-1) + NADP+ | - |
? | |
polysulfide(n) + NADPH + H+ | NADH can not replace NADPH, the purified recombinant enzyme catalyzes the reduction of polysulfide with NADPH as an electron donor and it also reduces oxygen | Thermococcus litoralis DSM 5473 | hydrogen sulfide + polysulfide(n-1) + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at, the temperature optimum of the enzyme can not be determined due to the significant thermolability of NADPH above 80°C | Thermococcus litoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
assay at | Thermococcus litoralis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the purified recombinant NsoC contains one FAD cofactor per protein molecule | Thermococcus litoralis | |
NADPH | NADH can not replace NADPH | Thermococcus litoralis |