Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme can be reactivated by molybdenum and dithioerythritol | Neurospora crassa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | nit-1 mutant: lacks all activities except FAD-dependent NADPH:cytochrome c reductase activity,nit-2 mutant: reduced FAD:- and reduced methyl viologen:nitrate reductase activities but lacks the other two activities | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | 1 mol per mol enzyme protein, activation of enzyme after urea treatment, little stimulation | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
132000 | - |
2 * 97000: nit-3 enzyme, 2 * 132000, wild-type | Neurospora crassa |
204000 | - |
nit-3 enzyme, gel filtration | Neurospora crassa |
272000 | - |
wild-type, gel filtration | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | Neurospora crassa | first step in nitrate assimilation | nitrite + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
wild-type STA4, nit-1 and nit-3 mutants | - |
Purification (Comment) | Organism |
---|---|
partially purification of wild-type and nit-3 mutant by streptomycin sulfate precipitation and ammonium sulfate fractionation, of nit-1 mutant by protamine sulfate fractionation, ammonium sulfate precipitation and gel filtration | Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Neurospora crassa | - |
Storage Stability | Organism |
---|---|
5°C, urea-treated, 0.1 M potassium phosphate buffer, pH 7.3, 5 mM EDTA, 2 mM dithioerythritol, 0.5 mg/ml or more bovine serum albumin, stable for 18 h without loss of activity | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | first step in nitrate assimilation | Neurospora crassa | nitrite + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 97000: nit-3 enzyme, 2 * 132000, wild-type | Neurospora crassa |
More | nit-1 mutant enzyme is the apoprotein of nitrate reductase | Neurospora crassa |
More | aggregate of two different polypeptide chains: one responsible for transport of electrons fom NADPH to FAD or cytochrome c, nit-1 enzyme. The other transfers electrons from FAD via molybdenum to nitrate, nit-3 enzyme | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b557 | - |
Neurospora crassa | |
heme | 2 mol heme iron per mol enzyme protein | Neurospora crassa |