Cloned (Comment) | Organism |
---|---|
expression of wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzymes in Escherichia coli | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
H49A | reduced kcat | Sus scrofa |
H49E | elevated Km value for cytochrome b5, strongly reduced kcat | Sus scrofa |
H49K | reduced Km value for cytochrome b5 | Sus scrofa |
H49Y | similar to wild-type | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
NADH | H49E mutant enzyme | Sus scrofa | |
0.0006 | - |
ferricyanide | H49K mutant enzyme | Sus scrofa | |
0.00064 | - |
NADH | G273 mutant enzyme | Sus scrofa | |
0.00084 | - |
NADH | DELTAF272 mutant enzyme | Sus scrofa | |
0.0015 | - |
NADH | recombinant wild-type enzyme | Sus scrofa | |
0.0016 | - |
NADH | native enzyme | Sus scrofa | |
0.0019 | - |
NADH | H49A mutant enzyme | Sus scrofa | |
0.0022 | - |
ferricyanide | H49E mutant enzyme | Sus scrofa | |
0.0025 | - |
ferricyanide | native enzyme | Sus scrofa | |
0.0025 | - |
cytochrome b5 | G273 mutant enzyme | Sus scrofa | |
0.0026 | - |
ferricyanide | H49Y mutant enzyme | Sus scrofa | |
0.0027 | - |
NADH | H49Y mutant enzyme | Sus scrofa | |
0.0028 | - |
ferricyanide | recombinant wild-type enzyme | Sus scrofa | |
0.0028 | - |
ferricyanide | H49A mutant enzyme | Sus scrofa | |
0.0031 | - |
cytochrome b5 | H49K mutant enzyme | Sus scrofa | |
0.0052 | - |
ferricyanide | DELTAF272 mutant enzyme | Sus scrofa | |
0.0052 | - |
ferricyanide | G273 mutant enzyme | Sus scrofa | |
0.0088 | - |
cytochrome b5 | native enzyme | Sus scrofa | |
0.0089 | - |
cytochrome b5 | H49A mutant enzyme | Sus scrofa | |
0.0091 | - |
cytochrome b5 | recombinant wild-type enzyme | Sus scrofa | |
0.01 | - |
NADH | H49K mutant enzyme | Sus scrofa | |
0.011 | - |
cytochrome b5 | H49Y mutant enzyme | Sus scrofa | |
0.017 | - |
cytochrome b5 | DELTAF272 mutant enzyme | Sus scrofa | |
0.03 | - |
cytochrome b5 | H49E mutant enzyme | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzyme | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + ferricytochrome b5 | - |
Sus scrofa | NAD+ + H+ + ferrocytochrome b5 | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
53 | - |
NADH | G273 mutant enzyme | Sus scrofa | |
57 | - |
ferricytochrome b5 | G273 mutant enzyme | Sus scrofa | |
76 | - |
ferricyanide | G273 mutant enzyme | Sus scrofa | |
77 | - |
NADH | H49E mutant enzyme | Sus scrofa | |
110 | - |
ferricyanide | H49E mutant enzyme | Sus scrofa | |
160 | - |
ferricytochrome b5 | H49E mutant enzyme | Sus scrofa | |
310 | - |
NADH | DELTAF272 mutant enzyme | Sus scrofa | |
380 | - |
ferricytochrome b5 | H49A mutant enzyme | Sus scrofa | |
390 | - |
NADH | native enzyme | Sus scrofa | |
400 | - |
ferricyanide | DELTAF272 mutant enzyme | Sus scrofa | |
420 | - |
ferricytochrome b5 | H49K mutant enzyme | Sus scrofa | |
440 | - |
NADH | H49A mutant enzyme | Sus scrofa | |
450 | - |
ferricytochrome b5 | DELTAF272 mutant enzyme | Sus scrofa | |
470 | - |
ferricyanide | H49A mutant enzyme | Sus scrofa | |
490 | - |
ferricyanide | H49K mutant enzyme | Sus scrofa | |
510 | - |
ferricytochrome b5 | native enzyme | Sus scrofa | |
560 | - |
ferricytochrome b5 | recombinant wild-type enzyme | Sus scrofa | |
570 | - |
NADH | recombinant wild-type enzyme | Sus scrofa | |
580 | - |
ferricytochrome b5 | H49Y mutant enzyme | Sus scrofa | |
630 | - |
ferricyanide | native enzyme | Sus scrofa | |
710 | - |
NADH | H49K mutant enzyme | Sus scrofa | |
727 | - |
ferricyanide | H49Y mutant enzyme | Sus scrofa | |
750 | - |
NADH | H49Y mutant enzyme | Sus scrofa | |
810 | - |
ferricyanide | recombinant wild-type enzyme | Sus scrofa |