Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-hydroxymercuribenzoate | 0.1 mM, complete inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
methemoglobin-ferrocyanide complex | - |
Oryctolagus cuniculus | |
0.01 | - |
ferricyanide | - |
Oryctolagus cuniculus | |
0.015 | - |
cytochrome b5 | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
liver, cytosol, gel filtration, sucrose density gradient centrifugation | Oryctolagus cuniculus |
30000 | - |
enzyme from liver cytosol, gel filtration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.69 | - |
methemoglobin-ferrocyanide reductases assay | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-20°C, several months, no loss of activity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | additional acceptor: methemoglobin-ferrocyanide complex | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
methemoglobin-ferrocyanide complex + NADH | - |
Oryctolagus cuniculus | reduced methemoglobin-ferrocyanide complex + NAD+ | - |
? | |
NADH + ferricyanide | - |
Oryctolagus cuniculus | NAD+ + H+ + ferrocyanide | - |
r | |
NADH + ferricytochrome b5 | - |
Oryctolagus cuniculus | NAD+ + H+ + ferrocytochrome b5 | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
Tris-phosphate buffer | Oryctolagus cuniculus |
7 | - |
triethanolamine buffer | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Oryctolagus cuniculus |