Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a therapeutic target for the treatment of primary hyperoxaluria | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1R,3R)-3-hydroxy-cyclopentane-carboxylate | - |
Homo sapiens | |
5-hydroxy-1H-pyrazole-3-carboxylate | - |
Homo sapiens | |
menadione | - |
Homo sapiens | |
additional information | (1S,3R)-3-hydroxy-cyclopentane carboxylic acid, trans-4-hydroxy-D-proline, cis-4-hydroxy-L-proline, and cis-4-hydroxy-D-proline do not cause inhibition even up to 5 mM | Homo sapiens | |
Tetrahydrofuroic acid | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
menaquinone | at pH 7.5 and 25°C | Homo sapiens | |
0.124 | - |
coenzyme Q1 | at pH 7.5 and 25°C | Homo sapiens | |
0.143 | - |
duroquinone | at pH 7.5 and 25°C | Homo sapiens | |
200 | - |
trans-4-hydroxy-L-proline | at pH 7.5 and 25°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-4-hydroxy-L-proline + coenzyme Q10 | Homo sapiens | preferred substrate | (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UF12 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and HisPur cobalt resin column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + coenzyme Q1 | - |
Homo sapiens | ? | - |
? | |
additional information | no activity with trans-4-hydroxy-D-proline, cis-4-hydroxy-L-proline, and cis-4-hydroxy-D-proline | Homo sapiens | ? | - |
? | |
additional information | the enzyme is a poor oxidase using O2 as electron acceptor | Homo sapiens | ? | - |
? | |
trans-4-hydroxy-L-proline + coenzyme Q1 | - |
Homo sapiens | (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ? | - |
? | |
trans-4-hydroxy-L-proline + coenzyme Q10 | preferred substrate | Homo sapiens | (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + ? | - |
? | |
trans-4-hydroxy-L-proline + duroquinone | - |
Homo sapiens | (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + duroquinol | - |
? | |
trans-4-hydroxy-L-proline + menadione | - |
Homo sapiens | (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + menadiol | - |
? | |
trans-4-hydroxy-L-proline + menaquinone | - |
Homo sapiens | (3R,5S)-3-hydroxy-1-pyrroline-5-carboxylate + menaquinol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hydroxyproline oxidase | - |
Homo sapiens |
HYPDH | - |
Homo sapiens |
ProDH2 | - |
Homo sapiens |
proline dehydrogenase 2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
coenzyme Q1 | at pH 7.5 and 25°C | Homo sapiens | |
0.19 | - |
trans-4-hydroxy-L-proline | at pH 7.5 and 25°C | Homo sapiens | |
0.27 | - |
duroquinone | at pH 7.5 and 25°C | Homo sapiens | |
0.28 | - |
menaquinone | at pH 7.5 and 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.306 | - |
menadione | at pH 7.5 and 25°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.5 | - |
at pH 7.5 and 25°C | Homo sapiens | Tetrahydrofuroic acid | |
2.1 | - |
at pH 7.5 and 25°C | Homo sapiens | (1R,3R)-3-hydroxy-cyclopentane-carboxylate | |
2.9 | - |
at pH 7.5 and 25°C | Homo sapiens | 5-hydroxy-1H-pyrazole-3-carboxylate |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.93 | - |
trans-4-hydroxy-L-proline | at pH 7.5 and 25°C | Homo sapiens | |
1.5 | - |
coenzyme Q1 | at pH 7.5 and 25°C | Homo sapiens | |
1.9 | - |
duroquinone | at pH 7.5 and 25°C | Homo sapiens | |
11 | - |
menaquinone | at pH 7.5 and 25°C | Homo sapiens | |
75 | - |
L-proline | at pH 7.5 and 25°C | Homo sapiens |