Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | optimal dehydrogenase activity requires 15 mM or higher concentrations | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of FDH in insect cells using the pVL 1393 baculovirus expression vector | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | recombinant FDH is not inhibited by sodium azide | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
4 * 106000, SDS-PAGE | Rattus norvegicus |
450000 | - |
nondenaturing PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | Rattus norvegicus | regulatory mechanism to control the in vivo folate pool size | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of recombinant liver FDH | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.094 | - |
recombinant FDH, 10-formyltetrahydrofolate as substrate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-5,8-dideazafolate + NADP+ + H2O | - |
Rattus norvegicus | 5,8-dideazafolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | - |
Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | regulatory mechanism to control the in vivo folate pool size | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
additional information | enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate | Rattus norvegicus | ? | - |
? | |
additional information | FDH has also methyltransferase activity | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 106000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | identical with protein-arginine methyltransferase from liver cytosol, EC 2.1.1.23 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
folate | 10-formyl-5,8-dideazafolate and 5,8-dideazafolate | Rattus norvegicus | |
NADP+ | NADP+-dependent | Rattus norvegicus | |
tetrahydrofolate | - |
Rattus norvegicus |