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Literature summary for 1.5.1.6 extracted from

  • Krupenko, S.A.; Wagner, C.; Cook, R.J.
    Recombinant 10-formyltetrahydrofolate dehydrogenase catalyses both dehydrogenase and hydrolase reactions utilizing the synthetic substrate 10-formyl-5,8-dideazafolate (1995), Biochem. J., 306, 651-655.
No PubMed abstract available
Search for more literature for 1.5.1.6

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol dehydrogenase and hydrolase activities are dependent on the presence of high concentrations of 2-mercaptoethanol to prevent the oxidative degradation of the substrate 10-formyltetrahydrofolate Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the cDNA encoding FDH Rattus norvegicus
expression of FDH cDNA, subcloned in the pVL 1393 baculovirus vector, in Sf9 insect cells, using the MaxBac expression system Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00086
-
 NADP+ with 10-formyl-5,8-dideazafolate as substrate Rattus norvegicus
0.00088
-
 NADP+ with 10-formyltetrahydrofolate as substrate Rattus norvegicus
0.0032
-
 10-formyl-5,8-dideazafolate
-
 Rattus norvegicus
0.0055
-
 10-formyltetrahydrofolate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O Rattus norvegicus natural substrate tetrahydrofolate + CO2 + NADPH + H+
-
 ?
10-formyltetrahydropteroylpentaglutamate + NADP+ + H2O Rattus norvegicus natural substrate tetrahydropteroylpentaglutamate + CO2 + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.144
-
 with 10-formyl-5,8-dideazafolate as substrate Rattus norvegicus
0.26
-
 with 10-formyltetrahydrofolate as substrate Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyl-5,8-dideazafolate + NADP+ + H2O good substrate Rattus norvegicus 5,8-dideazafolate + CO2 + NADPH + H+
-
 ?
10-formyl-5,8-dideazafolate + NADP+ + H2O stable synthetic analogue can substitute for the labile natural substrate, affinity is twice as high as for 10-formyltetrahydrofolate Rattus norvegicus 5,8-dideazafolate + CO2 + NADPH + H+
-
 ?
10-formyltetrahydrofolate + NADP+ + H2O
-
 Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
10-formyltetrahydrofolate + NADP+ + H2O natural substrate Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
10-formyltetrahydropteroylpentaglutamate + NADP+ + H2O natural substrate Rattus norvegicus tetrahydropteroylpentaglutamate + CO2 + NADPH
-
 ?
additional information bifunctional enzyme: NADP+-dependent dehydrogenase activity and NADP+-independent hydrolase activity of 10-formyltetrahydrofolate with two active sites Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
10-formyltetrahydrofolate 10-formyltetrahydrofolate is one of the most labile of the reduced coenzymes Rattus norvegicus
folate 10-formyl-5,8-dideazafolate and 5,8-dideazafolate Rattus norvegicus
NADP+ NADP+-dependent Rattus norvegicus
tetrahydrofolate
-
 Rattus norvegicus