Cloned (Comment) | Organism |
---|---|
cloning of the cDNA encoding FDH | Rattus norvegicus |
cloning of the cDNA encoding FDH, cells from several tumor cell lines are transfected with FDH cDNA cloned into pcDNA 3.1+ vector using LipofectAMINE, expression of FDH inhibits the proliferation of the cell lines as a result of its enzymatic activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C707A | - |
Rattus norvegicus |
C707A | catalytically inactive mutant | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | Rattus norvegicus | - |
tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | Homo sapiens | regulation of the ratio of 10-formyltetrahydrofolate to tetrahydrofolate and of the purine biosynthesis through control of the level of 10-formyltetrahydrofolate, down-regulation of FDH in tumors is one of the cellular mechanisms that enhance proliferation, FDH possesses tumor specific suppressor effects | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
male rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | significantly decreased levels of FDH and its mRNA in 15 tested tumors compared with normal tissues, strong down-regulation of FDH on both the mRNA and protein level in tumors | Homo sapiens | - |
brain | moderate FDH mRNA level | Homo sapiens | - |
DU-145 cell | - |
Homo sapiens | - |
heart | - |
Rattus norvegicus | - |
heart | moderate FDH mRNA level | Homo sapiens | - |
HEK-293 cell | transformed embryonic kidney cell line HEK-293A | Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
kidney | high FDH mRNA level, specialized organ of folate metabolism | Homo sapiens | - |
liver | specialized organ of folate metabolism | Homo sapiens | - |
liver | high FDH mRNA level | Homo sapiens | - |
liver | high FDH mRNA level | Rattus norvegicus | - |
lung | moderate FDH mRNA level | Homo sapiens | - |
additional information | FDH expression is highly tissue specific, very low or nondetectable levels of FDH mRNA in placenta, spleen, colon, small intestine, and leukocytes | Homo sapiens | - |
muscle | moderate FDH mRNA level in skeletal muscle | Homo sapiens | - |
ovary | moderate FDH mRNA level | Homo sapiens | - |
pancreas | high FDH mRNA level | Homo sapiens | - |
PC-3 cell | - |
Homo sapiens | - |
PPC-1 cell | - |
Homo sapiens | - |
prostate gland | moderate FDH mRNA level | Homo sapiens | - |
testis | moderate FDH mRNA level | Homo sapiens | - |
thymus | moderate FDH mRNA level | Homo sapiens | - |
Tsu-Pr1 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | - |
Homo sapiens | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | - |
Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | regulation of the ratio of 10-formyltetrahydrofolate to tetrahydrofolate and of the purine biosynthesis through control of the level of 10-formyltetrahydrofolate, down-regulation of FDH in tumors is one of the cellular mechanisms that enhance proliferation, FDH possesses tumor specific suppressor effects | Homo sapiens | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Homo sapiens | |
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
NADP+ | - |
Homo sapiens | |
NADP+ | - |
Rattus norvegicus | |
tetrahydrofolate | - |
Homo sapiens | |
tetrahydrofolate | - |
Rattus norvegicus |