Application | Comment | Organism |
---|---|---|
medicine | formic acidemia: toxicity of methanol in humans is correlated with formate accumulation as a result of low rates of formate oxidation, which is in vivo dependent on enzyme activity and on hepatic tetrahydrofolate levels | Homo sapiens |
General Stability | Organism |
---|---|
in general activity is labile | Homo sapiens |
in general activity is labile | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
10-formyltetrahydrofolate | - |
Rattus norvegicus | |
0.05 | - |
10-formyltetrahydrofolate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
x * 96000, SDS-PAGE | Homo sapiens |
96000 | - |
x * 96000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | Rattus norvegicus | - |
tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | Homo sapiens | folate-dependent pathway is the exclusive route of formate metabolism in primates, plays major role in disposition of formate generated from one-carbon moieties of environmental chemicals, drugs, and foods, importance in the toxicity of methanol | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
Sprague-Dawley rats | - |
Purification (Comment) | Organism |
---|---|
free of 10-formyltetrahydrofolate hydrolase activity | Homo sapiens |
free of 10-formyltetrahydrofolate hydrolase activity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | activity in human liver is 26% of that in rat liver, reduced amount of enzyme in human liver | Homo sapiens | - |
liver | activity in human liver is 26% of that in rat liver, reduced amount of enzyme in human liver | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | - |
Homo sapiens | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | - |
Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | folate-dependent pathway is the exclusive route of formate metabolism in primates, plays major role in disposition of formate generated from one-carbon moieties of environmental chemicals, drugs, and foods, importance in the toxicity of methanol | Homo sapiens | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 96000, SDS-PAGE | Homo sapiens |
? | x * 96000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay at room temperature | Homo sapiens |
additional information | - |
assay at room temperature | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Homo sapiens | |
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
NADP+ | - |
Homo sapiens | |
NADP+ | - |
Rattus norvegicus | |
tetrahydrofolate | - |
Homo sapiens | |
tetrahydrofolate | - |
Rattus norvegicus |