Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | - |
Rattus norvegicus | |
tetrahydrofolate | - |
Sus scrofa | |
tetrahydropteroylhexaglutamate | 1 mol per mol of tetrameric protein, potent product inhibitor: 0.0003 mM, 90% inhibition | Sus scrofa | |
tetrahydropteroylpolyglutamate | potent product inhibitor | Rattus norvegicus | |
tetrahydropteroylpolyglutamate | inhibition increases with increasing length of polyglutamyl chain; potent product inhibitor | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Sus scrofa | |
0.007 | - |
10-formyltetrahydrofolate | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
4 * 91000, three minor bands of 63000, 58000 and 39000, SDS-PAGE | Sus scrofa |
390000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | Rattus norvegicus | disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | Sus scrofa | disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male sprague-dawley rats | - |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.51 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | - |
Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | very tight binding of product, binds its product rather than its substrate | ? | |
10-formyltetrahydrofolate + NADP+ + H2O | - |
Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | very tight binding of product, binds its product rather than its substrate | ? | |
10-formyltetrahydrofolate + NADP+ + H2O | disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism | Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydropteroylhexaglutamate + NADP+ + H2O | - |
Sus scrofa | tetrahydropteroylhexaglutamate + CO2 + NADPH | - |
? | |
10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O | - |
Rattus norvegicus | tetrahydropteroylpolyglutamate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O | affinity increases with increasing length of polyglutamyl chain | Sus scrofa | tetrahydropteroylpolyglutamate + CO2 + NADPH + H+ | - |
? | |
additional information | bifunctional protein: 10-formyltetrahydrofolate dehydrogenase/hydrolase, hydrolysis at 20-30% of the oxidative rate | Rattus norvegicus | ? | - |
? | |
additional information | bifunctional protein: 10-formyltetrahydrofolate dehydrogenase/hydrolase, hydrolysis at 20-30% of the oxidative rate | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Rattus norvegicus |
homotetramer | 4 * 91000, three minor bands of 63000, 58000 and 39000, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
More | identical with folate binding protein cytosol-I, FBP-CI | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Sus scrofa | |
NADP+ | - |
Rattus norvegicus | |
NADP+ | - |
Sus scrofa | |
tetrahydrofolate | very tight binding | Rattus norvegicus | |
tetrahydrofolate | very tight binding | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
tetrahydrofolate | - |
Sus scrofa | |
0.007 | - |
tetrahydrofolate | - |
Rattus norvegicus |