Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Vibrio harveyi |
Protein Variants | Comment | Organism |
---|---|---|
E99K | the mutation destabilizes the dimer and has an enhanced subunit dissociation as evident from a 44fold higher Kd for the monomer-dimer equilibrium | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00007 | - |
NADPH | mutant enzyme E99K, in 50 mM phosphate buffer, at pH 7.0, at 23°C | Vibrio harveyi | |
0.002 | - |
FMN | mutant enzyme E99K, in 50 mM phosphate buffer, at pH 7.0, at 23°C | Vibrio harveyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26300 | - |
2 * 26300 | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultracentrifugation, G-25 gel filtration, DEAE-Sepharose column chromatography, and Sephadex G25 gel filtration | Vibrio harveyi |
Renatured (Comment) | Organism |
---|---|
soluble denatured mutant enzyme E99K is mixed into 50fold volume of pre-cooled 50 mM phosphate buffer containing 0.1 M FMN and stirred on ice in the dark for 15 min for protein renaturation and the reconstitution of E99K holoenzyme | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26300 | Vibrio harveyi |
Synonyms | Comment | Organism |
---|---|---|
FRP | - |
Vibrio harveyi |
NADPH-specific flavin reductase | - |
Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | contains one bound FMN cofactor per subunit | Vibrio harveyi | |
NADPH | - |
Vibrio harveyi |