Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
both the chaperone systems GroEL (minichaperone) and GroEL-GroES prevent thermal aggregation and assist in refolding of DHFR | Danio rerio |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
half-life 5.18 min, in presence of GroEL (minichaperone) half-life 12.35 min, in presence of GroEL-GroES half-life 21.32 min | Danio rerio |
50 | - |
complete unfolding and loss of activity. The heat induced inactivation follows first-order kinetics and Arrhenius law | Danio rerio |
50 | - |
half-life 2.13 min, in presence of GroEL (minichaperone) half-life 8.09 min, in presence of GroEL-GroES half-life 16.54 min | Danio rerio |
55 | - |
half-life 1.33 min, in presence of GroEL (minichaperone) half-life 3.44 min, in presence of GroEL-GroES half-life 7.19 min | Danio rerio |