Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | up to 20% increase in activity up to 0.2 M, activity declines above and falls to zero at 0.8 M | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | up to 20% increase in activity up to 0.2 M, activity declines above and falls to zero at 0.8 M | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
7,8-dihydrofolate | pH 7.0, 60°C | Geobacillus stearothermophilus | |
0.0104 | - |
NADPH | pH 7.0, 60°C | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
gel filtration | Geobacillus stearothermophilus |
18694 | - |
1 * 18694, electrospray mass spectrometry and calculated, 1 * 20000, SDS-PAGE | Geobacillus stearothermophilus |
20000 | - |
1 * 18694, electrospray mass spectrometry and calculated, 1 * 20000, SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | ordered kinetic mechanism at elevated temperature around 60°C, with NADPH binding first. More random mechanism at temperatures around 20°C with greatly reduced Km-value for 7,8-dihydrofolate. Rate-limiting hydride-transfer with a moderate enthalpy of activiation | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Geobacillus stearothermophilus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 18694, electrospray mass spectrometry and calculated, 1 * 20000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
at pH 9 | Geobacillus stearothermophilus |
75 | - |
at pH 7 | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
in presence of 5fold excess of NADPH or substrate, 70-80% residual activity after 1 h | Geobacillus stearothermophilus |
64 | - |
rapid loss of activity in absence of substrate above 64°C | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
218 | - |
NADPH | pH 7.0, 60°C | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Geobacillus stearothermophilus | |
NADPH | - |
Geobacillus stearothermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus stearothermophilus | isoelectric focusing | - |
8.2 |