Protein Variants | Comment | Organism |
---|---|---|
S209A | site-directed mutagenesis of the phosphorylation site. The MAO A S209A mutant exhibits similar catalytic properties to those of wild-type enzyme, its catalytic activity is reduced by 1.5-3.7fold compared to the wild-type MAO A | Homo sapiens |
S209E | site-directed mutagenesis of the phosphorylation site. Compared with purified wild-type and S209A MAO A proteins, the S209G MAO A mutant shows 10fold reduced kcat values and 10fold increased Km values compared to the wild-type enzymesignificant differences in covalent flavin fluorescence yield, circular dichroism spectra and thermal stability | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Clorgyline | an acetylenic inhibitor, addition of it results in the conversion of the oxidized flavin cofactors to their respective N(5) flavocyanine adducts | Homo sapiens | |
D-amphetamine | a nonselective reversible MAO inhibitor, inhibits the MAO A S209E mutant with much lower affinity, 160fold and 20fold, respectively, than the wild-type enzyme | Homo sapiens | |
Harmane | an MAO A specific reversible inhibitor | Homo sapiens | |
isatin | a nonselective reversible MAO inhibitor, inhibits the MAO A S209E mutant with much lower affinity, 160fold and 20fold, respectively, than the wild-type enzyme | Homo sapiens | |
phentermine | binding to the S209E mutant is reduced by 13fold compatred to the wild-type MAO A | Homo sapiens | |
pirlindole | an MAO A specific reversible inhibitor | Homo sapiens | |
tetrindole | an MAO A specific reversible inhibitor | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial outer membrane | - |
Homo sapiens | 5741 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P21397 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
flavoprotein | - |
Homo sapiens |
phosphoprotein | MAO A is phosphorylated at Ser209, which does not play a role in enzyme regulation, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + H2O + O2 | MAO-A | Homo sapiens | benzaldehyde + NH3 + H2O2 | - |
? | |
kynuramine + H2O + O2 | MAO-A | Homo sapiens | 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2 | - |
? | |
additional information | structure-activity studies on wild-type and mutant enzymes, active site structure, overview | Homo sapiens | ? | - |
? | |
phenylethylamine + H2O + O2 | MAO-A | Homo sapiens | 2-phenylethanal + NH3 + H2O2 | - |
? | |
serotonin + H2O + O2 | MAO-A | Homo sapiens | (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure-activity studies on wild-type and mutant enzymes, active site structure, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MAO A | - |
Homo sapiens |
monoamine oxidase A | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | comparison of flavin fluorescence and circular dichroism spectral properties of wild-type MAO A and of the MAO A Ser209 mutant proteins | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
6.682 | - |
phentermine | - |
Homo sapiens |