Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Platichthys stellatus |
Crystallization (Comment) | Organism |
---|---|
homology modeling of LAAO1 predicts metal coordination by residues Y241, H348, and D406 | Platichthys stellatus |
Protein Variants | Comment | Organism |
---|---|---|
H209A | non-metal coordinating mutant, the levels of iron, zinc, and magnesium are similar to those of wild-type | Platichthys stellatus |
H348A | contrary to wild-type, no magnesium ion is detected | Platichthys stellatus |
H348A | mutant does not display antibacterial activity | Platichthys stellatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | the Michaelis constant of LAAO1 decreases and the catalytic constant (Kcat/Km) value increases following pre-treatment with EDTA. EDTA pre-chelated LAAO1 is more active than the non-EDTA treated LAAO1 sample against Staphylococcus aureus ATCC 25923, MRSA clinical isolate 87-7927, and Vibrio parahaemolyticus RIMD2210001 | Platichthys stellatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
L-lysine | pH 7, 37°C, EDTA-treated protein | Platichthys stellatus | |
67.7 | - |
L-lysine | pH 7, 37°C, untreated protein | Platichthys stellatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the mole ratios of one wild-type protein to iron, zinc, and magnesium are 0.12, 0.50, and 3.61, respectively | Platichthys stellatus | |
Magnesium | the mole ratios of one wild-type protein to iron, zinc, and magnesium are 0.12, 0.50, and 3.61, respectively | Platichthys stellatus | |
Zinc | the mole ratios of one wild-type protein to iron, zinc, and magnesium are 0.12, 0.50, and 3.61, respectively | Platichthys stellatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Platichthys stellatus | D2KWB6 | isoform LAAO-1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + H2O + O2 | - |
Platichthys stellatus | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAAO-1 | - |
Platichthys stellatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00085 | - |
L-lysine | pH 7, 37°C, EDTA-treated protein | Platichthys stellatus | |
0.0302 | - |
L-lysine | pH 7, 37°C, untreated protein | Platichthys stellatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
L-lysine | pH 7, 37°C, untreated protein | Platichthys stellatus | |
0.00085 | - |
L-lysine | pH 7, 37°C, EDTA-treated protein | Platichthys stellatus |