Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminobenzoic acid | three and two binding sites in isoforms L1 and L2, respectively | Daboia russelii russelii | |
additional information | initial uncompetitive inhibition of L1 followed by mixed inhibition at higher concentrations suggesting the existence of two different inhibitor-binding sites distinct from the substrate-binding site. In the case of L2, initial linear competitive inhibition followed by mixed inhibition suggesting the existence of two nonoverlapping inhibitor-binding sites, one of which is the substrate-binding site | Daboia russelii russelii | |
N-acetyl-L-tryptophan amide | inhibition of both isoforms L1 and L2 | Daboia russelii russelii | |
N-acetyltryptophan | inhibition of both isoforms L1 and L2 | Daboia russelii russelii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00144 | - |
L-isoleucine | isoform L1 | Daboia russelii russelii | |
0.00189 | - |
L-isoleucine | isoform L2 | Daboia russelii russelii | |
0.0493 | - |
L-phenylalanine | isoform L2 | Daboia russelii russelii | |
0.052 | - |
L-tyrosine | isoform L1 | Daboia russelii russelii | |
0.0665 | - |
L-phenylalanine | isoform L1 | Daboia russelii russelii | |
0.211 | - |
L-tryptophan | isoform L1 | Daboia russelii russelii | |
0.223 | - |
L-methionine | isoform L2 | Daboia russelii russelii | |
0.235 | - |
L-tryptophan | isoform L2 | Daboia russelii russelii | |
0.297 | - |
L-methionine | isoform L1 | Daboia russelii russelii | |
0.538 | - |
L-tyrosine | isoform L2 | Daboia russelii russelii | |
0.6 | - |
L-leucine | isoform L2 | Daboia russelii russelii | |
0.75 | - |
L-leucine | isoform L1 | Daboia russelii russelii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daboia russelii russelii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration followed by ion exchange chromatography, to homogeneity | Daboia russelii russelii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Daboia russelii russelii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.94 | - |
isoform L2 | Daboia russelii russelii |
8.96 | - |
isoform L1 | Daboia russelii russelii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + H2O + O2 | - |
Daboia russelii russelii | 3-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-leucine + H2O + O2 | - |
Daboia russelii russelii | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | - |
Daboia russelii russelii | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | maximum catalytic efficiency for both isoforms | Daboia russelii russelii | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-tryptophan + H2O + O2 | - |
Daboia russelii russelii | 3-indole-2-oxopropanoate + NH3 + H2O2 | - |
? | |
L-tyrosine + H2O + O2 | - |
Daboia russelii russelii | p-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000-63000, purified isoforms L1 and L2, size exclusion HPLC and SDS-PAGE | Daboia russelii russelii |
Synonyms | Comment | Organism |
---|---|---|
LAAO | - |
Daboia russelii russelii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
both isoforms are stable up to 50°C but are sharply inactivated above 60°C | Daboia russelii russelii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.8 | both isoforms retain 100% activity, drastic inactivation above and below this range | Daboia russelii russelii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Daboia russelii russelii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01189 | - |
2-aminobenzoic acid | isoform L2, free enzyme | Daboia russelii russelii | |
0.02544 | - |
2-aminobenzoic acid | isoform L2, enzyme-substrate complex | Daboia russelii russelii | |
0.0935 | - |
N-acetyltryptophan | isoform L1, enzyme-substrate complex | Daboia russelii russelii | |
0.1146 | - |
N-acetyltryptophan | isoform L2, free enzyme | Daboia russelii russelii | |
0.1275 | - |
N-acetyltryptophan | isoform L1, free enzyme | Daboia russelii russelii | |
0.2001 | - |
N-acetyltryptophan | isoform L2, enzyme-substrate complex | Daboia russelii russelii | |
0.3788 | - |
N-acetyl-L-tryptophan amide | isoform L2, free enzyme | Daboia russelii russelii | |
0.3846 | - |
N-acetyl-L-tryptophan amide | isoform L1, enzyme-substrate complexe | Daboia russelii russelii | |
0.5916 | - |
N-acetyl-L-tryptophan amide | isoform L1, free enzyme | Daboia russelii russelii | |
0.7448 | - |
N-acetyl-L-tryptophan amide | isoform L2, enzyme-substrate complex | Daboia russelii russelii |