Application | Comment | Organism |
---|---|---|
additional information | expressed LAAO exhibits the same electrophoretic mobility as native LAAO and exhibits approximately the same extent of glycosylation as authentic LAAO from snake venom. Catalytic properties and substrate specificity of recombinant LAAO are similar to those of native enzyme | Calloselasma rhodostoma |
Cloned (Comment) | Organism |
---|---|
LAAO open reading frame inserted after the alpha-MF-signal sequence. Expressed in Pichia pastoris | Calloselasma rhodostoma |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tunicamycin | inhibits secretion of recombinant LAAO | Calloselasma rhodostoma |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Calloselasma rhodostoma | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Calloselasma rhodostoma |
62000 | - |
x * 62000, SDS-PAGE | Calloselasma rhodostoma |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Calloselasma rhodostoma | - |
- |
- |
Calloselasma rhodostoma | - |
expression in Pichia pastoris | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Calloselasma rhodostoma |
Purification (Comment) | Organism |
---|---|
by ion exchange and gel filtration, to homogeneity | Calloselasma rhodostoma |
recombinant enzyme | Calloselasma rhodostoma |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
supernatant from the culture medium | Calloselasma rhodostoma |
3.15 | - |
purified enzyme | Calloselasma rhodostoma |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + H2O + O2 | substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan | Calloselasma rhodostoma | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan | Calloselasma rhodostoma | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan | Calloselasma rhodostoma | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-tryptophan + H2O + O2 | substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan | Calloselasma rhodostoma | 3-indole-2-oxopropanoate + NH3 + H2O2 | - |
? | |
L-tyrosine + H2O + O2 | substrate affinity in decreasing order: L-methionine, L-leucine, L-phenylalanine, L-tyrosine, L-tryptophan | Calloselasma rhodostoma | p-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, SDS-PAGE | Calloselasma rhodostoma |
homodimer | 2 * 60000, SDS-PAGE | Calloselasma rhodostoma |
Synonyms | Comment | Organism |
---|---|---|
LAAO | - |
Calloselasma rhodostoma |