Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by pyridoxal 5-phosphate and pyridoxamine 5-phosphate | Euglena gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azaserine | completely inhibited by 1 mM, glutamine analog | Euglena gracilis | |
additional information | not inhibited by vitamin B6-enzyme inhibitors | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
2-oxoglutarate | - |
Euglena gracilis | |
0.02 | - |
NADH | - |
Euglena gracilis | |
0.3 | - |
L-glutamine | - |
Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Euglena gracilis | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
Z | - |
Euglena gracilis Z | - |
Z | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | absolute specificity for 2-oxoglutarate | Euglena gracilis | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | highly specific for its substrates | Euglena gracilis | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Euglena gracilis | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | absolute specificity for 2-oxoglutarate | Euglena gracilis Z | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | highly specific for its substrates | Euglena gracilis Z | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Euglena gracilis Z | L-glutamate + NAD+ | - |
? | |
additional information | no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH | Euglena gracilis | ? | - |
? | |
additional information | no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH | Euglena gracilis Z | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Euglena gracilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
rapid inactivation above | Euglena gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Euglena gracilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Euglena gracilis | |
NADH | specific for NADH as reductant | Euglena gracilis |