Application | Comment | Organism |
---|---|---|
medicine | inhibition of pyrimidine biosynthesis by targeting enzyme activity, mechanism for antimicrobial intervention | Enterococcus faecalis |
Cloned (Comment) | Organism |
---|---|
- |
Enterococcus faecalis |
expression in Escherichia coli BL21(DE3) TAP330, a pyrD(-) minus derivative | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Barbituric acid | competitive to dihydroorotate, dead-end inhibitor | Enterococcus faecalis | |
H2O2 | byproduct, tends to inactivate enzyme | Enterococcus faecalis | |
Orotate | - |
Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.115 | - |
S-dihydroorotate | - |
Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Enterococcus faecalis |
80000 | - |
gel filtration | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + acceptor | Enterococcus faecalis | reduction of a number of substrates: fumatate, coenzyme ubiquinone-0 and menadione | orotate + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Enterococcus faecalis | - |
family 1 enzyme A | - |
Purification (Comment) | Organism |
---|---|
streptomycin sulfate precipitation, anion-exchange, gel filtration | Enterococcus faecalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-dihydroorotate + a quinone = orotate + a quinol | 68% identity with type A enzyme of Lactococcus lactis, two-site non-classical ping-pong kinetic mechanism | Enterococcus faecalis |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 1 mM DTT, 0.01 mM FMN, no loss of activity during time of studies | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + acceptor | fumarate and menadione as electron acceptors | Enterococcus faecalis | orotate + reduced acceptor | - |
? | |
(S)-dihydroorotate + acceptor | reduction of a number of substrates: fumatate, coenzyme ubiquinone-0 and menadione | Enterococcus faecalis | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | acceptor: menadione | Enterococcus faecalis | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | acceptor: fumarate, coenzyme Q10 | Enterococcus faecalis | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | mechanism and pH-dependence of reaction | Enterococcus faecalis | orotate + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Enterococcus faecalis | |
FMN | flavoprotein | Enterococcus faecalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Barbituric acid | - |
Enterococcus faecalis | |
0.0388 | - |
Orotate | - |
Enterococcus faecalis |