Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Albifimbria verrucaria |
Protein Variants | Comment | Organism |
---|---|---|
M467Q | the enzymatic activity of the mutant is very low toward bilirubin but it works as a good catalyst in direct electron transfer-type bioelectrocatalytic reduction of dioxygen into water, the kcat value is 3fold increased | Albifimbria verrucaria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0192 | - |
K4[Fe(CN)6] | wild type recombinant enzyme, at pH 7.0 and 25°C | Albifimbria verrucaria | |
5.966 | - |
K4[Fe(CN)6] | mutant enzyme M467Q, at pH 7.0 and 25°C | Albifimbria verrucaria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | multi-copper oxidase | Albifimbria verrucaria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Albifimbria verrucaria | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Albifimbria verrucaria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bilirubin + O2 | - |
Albifimbria verrucaria | biliverdin + H2O | - |
? | |
K4[Fe(CN)6] + O2 | - |
Albifimbria verrucaria | K3[Fe(CN)6] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BOD | - |
Albifimbria verrucaria |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
374 | - |
K4[Fe(CN)6] | wild type recombinant enzyme, at pH 7.0 and 25°C | Albifimbria verrucaria | |
1259 | - |
K4[Fe(CN)6] | mutant enzyme M467Q, at pH 7.0 and 25°C | Albifimbria verrucaria |