Application | Comment | Organism |
---|---|---|
medicine | great potential for use as a diagnostic reagent, can be used for accurate determination of serum bilirubin levels in patients with hepatobiliary disease | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
Escherichia coli JM109 transformed with pUC119 carrying the CotA gene, produces large amounts of the soluble protein under low-temperature conditions | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
bilirubin | - |
Bacillus subtilis | |
0.015 | - |
ditaurobilirubin | - |
Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bilirubin + O2 | CotA with markedly higher affinity for bilirubin than conventional bilirubin oxidase | Bacillus subtilis | biliverdin + H2O | - |
? | |
ditaurobilirubin + O2 | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CotA | - |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | 37 | at 24°C approximately 50% of the CotA protein is detected in the soluble fraction | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable for 60 min | Bacillus subtilis |
84 | - |
stable for 30 min | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
optimal for ditaurobilirubin oxidation | Bacillus subtilis |
7 | - |
optimal for bilirubin oxidation | Bacillus subtilis |