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Literature summary for 1.3.3.3 extracted from
- Aerobic and anaerobic coproporphyrinogenase activities in extracts from Saccharomyces cerevisiae. Purification and characterization (1974), J. Biol. Chem., 249, 6367-6371.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-methionine | strictly required for anaerobic oxidation, optimal activation at 5 mM | Saccharomyces cerevisiae |  |
| pyruvate | 50% activation at 50 mM | Saccharomyces cerevisiae | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 25% inhibition of aerobic, 53% inhibition of anaerobic enzyme at 10 mM | Saccharomyces cerevisiae | |
| EDTA | 16% inhibition of aerobic, 41% inhibition of anaerobic enzyme at 10 mM | Saccharomyces cerevisiae | |
| GSH | slight inhibition of aerobic, 41% inhibition of anaerobic enzyme at 5 mM | Saccharomyces cerevisiae | |
| GSSG | 31% inhibition of anerobic, 25% inhibition of aerobic enzyme at 5 mM | Saccharomyces cerevisiae | |
| L-ethionine | inhibition of anaerobic enzyme, 48% at 5 mM | Saccharomyces cerevisiae | |
| additional information | decreased activity at high ionic strength | Saccharomyces cerevisiae | |
| N-ethylmaleimide | 86% inhibition of anaerobic, 54% inhibition of aerobic enzyme at 20 mM | Saccharomyces cerevisiae | |
| p-hydroxymercuribenzoate | 44% inhibition of anaerobic, 23% inhibition of aerobic enzyme at 0.5 mM | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
Coproporphyrinogen-III | same value for aerobic and anaerobic oxidation | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 50% activation of anaerobic enzyme at 5 mM | Saccharomyces cerevisiae | |
| Co2+ | optimal activation only for anaerobic enzyme at 0.1 mM | Saccharomyces cerevisiae | |
| Fe2+ | optimal activation only for anaerobic enzyme at 0.1 mM | Saccharomyces cerevisiae | |
| Fe3+ | 80% activation of anaerobic enzyme at 0.5 mM | Saccharomyces cerevisiae | |
| Mg2+ | 50% activation of anaerobic enzyme at 5 mM | Saccharomyces cerevisiae | |
| Mn2+ | optimal activation only for anaerobic enzyme at 0.2 mM | Saccharomyces cerevisiae | |
| additional information | 30% activation of aerobic oxidation by divalent ions | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coproporphyrinogen-III + 2 NADP+ | Saccharomyces cerevisiae | oxidation under anaerobic conditions strictly requires NADP+ as electron acceptor | protoporphyrinogen-IX + 2 CO2 + 2 NADPH + 2 H+ | - |
? | |
| coproporphyrinogen-III + O2 + 2 H+ | Saccharomyces cerevisiae | aerobic and anaerobic oxidation catalyzed by the same enzyme | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Saccharomyces cerevisiae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.007 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.05 M Tris-HCl, pH 7.6, 20% glycerol, 3 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coproporphyrinogen-III + 2 NADP+ | oxidation under anaerobic conditions strictly requires NADP+ as electron acceptor | Saccharomyces cerevisiae | protoporphyrinogen-IX + 2 CO2 + 2 NADPH + 2 H+ | - |
? | |
| coproporphyrinogen-III + O2 + 2 H+ | aerobic and anaerobic oxidation catalyzed by the same enzyme | Saccharomyces cerevisiae | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 7.7 | same value for anaerobic and aerobic oxidation | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | only for anaerobic activity, optimal activity at 2 mM | Saccharomyces cerevisiae | |
| NAD+ | only anaerobic activity, 30% of the activity compared to NADP+ | Saccharomyces cerevisiae | |
| NADP+ | strong specificity for anaerobic oxidation | Saccharomyces cerevisiae | |
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