Literature summary for 1.3.3.3 extracted from

Martasek, P.; Camadro, J.M.; Raman, R.S.; Lecomte, M.C.; Le Caer, J.P.; Demeler, B.; Grandchamp, B.; Labbe, P.
Human coproporphyrinogen oxidase. Biochemical characterization of recombinant normal and R231W mutated enzymes expressed in E. coli as soluble, catalytically active homodimers (1997), Cell. Mol. Biol., 43, 47-58.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
R231W	lowers Vmax-value, 4 fold lower catalytic efficiency	Homo sapiens

General Stability

General Stability	Organism
tends to aggregate during prolonged storage at 4°C	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0003	-	Coproporphyrinogen-III	-	Homo sapiens
0.0005	-	Coproporphyrinogen-III	mutant R231W	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no metal content	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	2 * 38000, SDS-PAGE	Homo sapiens
76000	-	gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
coproporphyrinogen-III + O2 + 2 H+	Homo sapiens	-	protoporphyrinogen-IX + 2 CO2 + 2 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, recombinant enzyme	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.07	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
coproporphyrinogen-III + O2 + 2 H+	-	Homo sapiens	protoporphyrinogen-IX + 2 CO2 + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 38000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
CPX	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	6.8	mutant R231W does not affect optimum	Homo sapiens

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Release 2023.1 (January 2023)