Protein Variants | Comment | Organism |
---|---|---|
T196A | mutant retains 1.0% of wild-type kcat | Mycobacterium tuberculosis |
T196V | mutant retains 0.15% of wild-type kcat | Mycobacterium tuberculosis |
Y158F | mutant retains only 1.8% of the wild-type kcat with a 2fold increase of the Km for octenoyl-CoA. Residue Y158 is essential for stereospecificity of protonation, the mutant accumulates a covalent adduct between crotonyl-CoA and NADH | Mycobacterium tuberculosis |
Y158S | strong decrease in kcat to only 0.16% of wild-type activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | mutant Y158F, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.056 | - |
NADH | mutant Y158S, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.059 | - |
NADH | mutant T196A, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.09 | - |
NADH | wild-type, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.11 | - |
NADH | mutant T196V, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.8 | - |
octenoyl-CoA | wild-type, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.9 | - |
octenoyl-CoA | mutant Y158S, pH 6.8, 30°C | Mycobacterium tuberculosis | |
2 | - |
octenoyl-CoA | mutant Y158F, pH 6.8, 30°C | Mycobacterium tuberculosis | |
4.3 | - |
octenoyl-CoA | mutant T196V, pH 6.8, 30°C | Mycobacterium tuberculosis | |
7.6 | - |
octenoyl-CoA | mutant T196A, pH 6.8, 30°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WGR1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WGR1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
octenoyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis | octanoyl-CoA + NAD+ | - |
? | |
octenoyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis H37Rv | octanoyl-CoA + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
NADH | mutant T196V, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.0055 | - |
octenoyl-CoA | mutant Y158S, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.0059 | - |
NADH | mutant Y158S, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.0073 | - |
octenoyl-CoA | mutant T196V, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.012 | - |
NADH | mutant T196A, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.05 | - |
octenoyl-CoA | mutant T196A, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.079 | - |
NADH | mutant Y158F, pH 6.8, 30°C | Mycobacterium tuberculosis | |
0.088 | - |
octenoyl-CoA | mutant Y158F, pH 6.8, 30°C | Mycobacterium tuberculosis | |
3.5 | - |
NADH | wild-type, pH 6.8, 30°C | Mycobacterium tuberculosis | |
3.6 | - |
octenoyl-CoA | wild-type, pH 6.8, 30°C | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the NADH cofactor and the CoA thioester substrate form a covalent adduct during the InhA catalytic cycle. Residue Tyr158 is required for the stereospecificity of protonation and Thr196 is partially involved in hydride transfer and protonation | Mycobacterium tuberculosis |