Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | no inhibition at 10 mM | Brassica napus | |
N-ethylmaleimide | no inhibition at 1 mM, 97% inhibition at 10 mM, inhibition can be partially reversed by dithiothreitol | Brassica napus | |
p-chloromercuribenzoate | complete inhibition at 0.1 mM, inhibition can be prevented by preincubation with crotonyl-CoA | Brassica napus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
crotonyl-[acyl-carrier protein] | Km is 0.001mM or less | Brassica napus | |
0.0076 | - |
NADH | - |
Brassica napus | |
0.178 | - |
crotonyl-CoA | - |
Brassica napus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33600 | - |
alpha2beta2, 2 *: 34800, 2 * 33600, SDS-PAGE | Brassica napus |
140000 | - |
gel filtration | Brassica napus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | - |
rape | - |
Purification (Comment) | Organism |
---|---|
homogeneity | Brassica napus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Brassica napus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200 | - |
- |
Brassica napus |
Storage Stability | Organism |
---|---|
-70°C, at least 3 months, survives only 1 freeze thaw cycle | Brassica napus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADH | - |
Brassica napus | butyryl-CoA + NAD+ | - |
? | |
crotonyl-[acyl-carrier protein] + NADH | - |
Brassica napus | butyryl-[acyl-carrier protein] + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2beta2, 2 *: 34800, 2 * 33600, SDS-PAGE | Brassica napus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
in 3-(N-morpholino)propane sulfonate buffer | Brassica napus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | - |
Brassica napus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | absolute specificity | Brassica napus |