General Stability | Organism |
---|---|
the crotonyl-CoA reductase activity is influenced by preincubation. 0.25 M Tris-HCl buffer diminishes the crotonyl-CoA reductase activity by approximately 30% | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | at high concentrations beta-mercaptoethanol is inhibitory | Bos taurus | |
dithiothreitol | at high concentrations dithiothreitol is inhibitory | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
trans-crotonyl-CoA | in 200 mM potassium phosphate buffer, pH 6.8, at 26°C | Bos taurus | |
0.00364 | - |
NADPH | in 200 mM potassium phosphate buffer, pH 6.8, at 26°C | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, calcium phosphate gel treatment, alumina gel Cgamma extract filtration, Sephadex G-200 gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mammary gland | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0061 | - |
crude extract, pH 6.8, at 26°C | Bos taurus |
0.0501 | - |
after 11.2fold purification, pH 6.8, at 26°C | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the crotonyl-CoA reductase reaction requires NADPH as electron donor, but at a 20fold higher concentration NADH will substitute for NADPH with 50% Vmax | Bos taurus | ? | - |
? | |
trans-crotonyl-CoA + NADPH + H+ | the crotonyl-CoA reductase reaction requires NADPH as electron donor | Bos taurus | butyryl-CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
crotonyl coenzyme A reductase | - |
Bos taurus |
trans-crotonyl CoA reductase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | the crotonyl-CoA reductase reaction requires NADPH as electron donor, but at a 20fold higher concentration NADH will substitute for NADPH with 50% Vmax | Bos taurus |