Cloned (Comment) | Organism |
---|---|
expression of xylL gene in Escherichia coli strain from TOL plasmid | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | Pseudomonas sp. | - |
catechol + CO2 + NADH | - |
? | |
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | Pseudomonas putida | - |
catechol + CO2 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
only TOL plasmid is mentioned, which is derived from Pseudomonas putida, but not Pseudomonas putida itself | - |
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | - |
Pseudomonas sp. | catechol + CO2 + NADH | - |
? | |
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | - |
Pseudomonas putida | catechol + CO2 + NADH | - |
? | |
3-methyl-4,5-cyclohexadiene-cis-1,2-diol-1-carboxylic acid + NAD+ | - |
Pseudomonas putida | 3-methylcatechol + CO2 + NADH | - |
? | |
additional information | purified recombinant enzyme also shows methyl transfer activity, methyl group is transferred from 4-methyl-3,5-cyclohexadiene-cis-1,2-diol-1-carboxylic acid to 3-methyl, only in presence of adenosylcobalamin as methyl group acceptor | Pseudomonas putida | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamin | required as methyl acceptor for the methyl transfer activity of recombinant enzyme from E. coli | Pseudomonas putida | |
NAD+ | - |
Pseudomonas sp. | |
NAD+ | - |
Pseudomonas putida |