Cloned (Comment) | Organism |
---|---|
expression of polyhistidine-tagged, codon-optimized cDNA in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-keto-5alpha-abiraterone | - |
Homo sapiens | |
3-keto-Delta4-abiraterone | - |
Homo sapiens | |
finasteride | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
progesterone | pH 6.0, 37°C | Homo sapiens | |
0.0003 | - |
progesterone | pH 7.4, 37°C | Homo sapiens | |
0.0008 | - |
testosterone | pH 6.0, 37°C | Homo sapiens | |
0.0014 | - |
androstenedione | pH 6.0, 37°C | Homo sapiens | |
0.003 | - |
3-keto-Delta4-abiraterone | pH 7.4, 37°C | Homo sapiens | |
0.0031 | - |
androstenedione | pH 7.4, 37°C | Homo sapiens | |
0.0061 | - |
17alpha-hydroxyprogesterone | pH 6.0, 37°C | Homo sapiens | |
0.0088 | - |
17alpha-hydroxyprogesterone | pH 7.4, 37°C | Homo sapiens | |
0.011 | - |
NADPH | pH 7.4, 37°C | Homo sapiens | |
0.0156 | - |
testosterone | pH 7.4, 37°C | Homo sapiens | |
0.016 | - |
NADPH | pH 6.0, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P18405 | isoform SRD5A1 | - |
Homo sapiens | P31213 | isoform SRD5A2 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein, use of detergents Triton X-100 and Nonidet P-40 for solubilization | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
functional reconstitution of recombinant enzyme, incorporation of the purified isoenzymes into Triton X-100-saturated dioleoylphosphatidylcholine liposomes and removal of excess detergent with polystyrene beads | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NADPH + H+ | - |
Homo sapiens | 17alpha-hydroxy-5alpha-pregnan-3,20-dione + NADP+ | - |
? | |
3-keto-delta4-abiraterone + NADPH + H+ | - |
Homo sapiens | ? + NADP+ | - |
? | |
androstenedione + NADPH + H+ | - |
Homo sapiens | 5alpha-androstan-3,17-dione + NADP+ | - |
? | |
progesterone + NADPH + H+ | - |
Homo sapiens | 5alpha-pregnan-3,20-dione + NADP+ | - |
? | |
testosterone + NADPH + H+ | - |
Homo sapiens | 5alpha-dihydrotestosterone + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, SDS-PAGE, x * 29000, calculated from sequence | Homo sapiens |
? | x * 22000, SDS-PAGE, x * 29000, calculated fromn sequence | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADH | Homo sapiens |