Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.20 extracted from

  • Bennett, M.J.; Albert, R.H.; Jez, J.M.; Ma, H.; Penning, T.M.; Lewis, M.
    Steroid recognition and regulation of hormone action: crystal structure of testosterone and NADP+ bound to 3alpha-hydroxysteroid/dihydrodiol dehydrogenase (1997), Structure, 5, 799-812.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
complexed with NADP+ and testosterone, 10fold molar excess of NADP+, 'waterbug' dialysis method in 10 mM potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM 2-mrcapthoethanol, 5% acetonitrile and 0.2 mM testosterone, and crystallization in presence of polethylene glycol 8000 in hanging drops at room temperature, single crystals Rattus norvegicus
cryocrystallization: crystals were soaked in 21% polyethylene glycol 8000, 0.1 M sodium cacodylate, pH 6.4, 1.25 mM NADP+, 0.1 mM testosterone and 15% 2-methyl-2,4-pentanediol as cryoprotectant Rattus norvegicus
structure determination, cofactor and substrate binding site, and model building Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37029
-
1 * 37029, sequence determination Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-dihydrotestosterone + NADPH + H+ Rattus norvegicus
-
5alpha-androstan-3alpha,17beta-diol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ structure analysis reveals that the enzyme belongs to the aldo-keto reductase superfamily, like 3alpha-hydroxysteroid dehydrogenase, 17beta-hydroxysteroid dehydrogenase, 20alpha-hydroxysteroid dehydrogenase, aldose reductase, and aldehyde reductase Rattus norvegicus
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ enzyme termed 3alpha-hydroxysteroid/dihydrodiol dehydrogenase, structure-based catalytic mechanism analysis, ordered bi-bi mechanism Rattus norvegicus
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ cofactor binding and catalytic triad, alpha/beta-barrel structure Rattus norvegicus
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ termed AKR1C as member of the aldo-keto-superfamily, overview Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-keto steroid + NAD(P)H
-
Rattus norvegicus 3alpha-hydroxy steroid + NAD(P)+
-
r
5alpha-dihydrotestosterone + NADPH + H+
-
Rattus norvegicus 5alpha-androstan-3alpha,17beta-diol + NADP+
-
r

Subunits

Subunits Comment Organism
monomer 1 * 37029, sequence determination Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Rattus norvegicus
NADPH
-
Rattus norvegicus