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Literature summary for 1.3.1.20 extracted from

  • Hara, A.; Harada, T.; Nakagawa, M.; Matsuura, K.; Nakayama, T.; Sawada, H.
    Isolation from pig lens of two proteins with dihydrodiol dehydrogenase and aldehyde reductase activities (1989), Biochem. J., 264, 403-407.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
diphosphate high concentration: F2 slight inhibition, F1 stimulation Sus scrofa
NaCl high concentration: F2 inhibition, F1 stimulation Sus scrofa
SO42- stimulates isoform F2 Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Barbital inhibits isoform F2 Sus scrofa
cyclopentane-1,1-diacetic acid NaCl inhibits only isoform F2 Sus scrofa
diphosphate high concentration: F2 slight inhibition, F1 stimulation Sus scrofa
Hexestrol i.e. 4,4'-(1,2-diethyl-1,2-ethanediyl)bisphenol, inhibits isoform F2 Sus scrofa
indomethacin inhibits both isoforms Sus scrofa
NaCl high concentration: F2 inhibition, F1 stimulation Sus scrofa
p-chloromercuribenzene sulfonate stronger effect on isoform F2 Sus scrofa
p-chloromercuribenzoate stronger effect on isoform F1 Sus scrofa
quercitrin inhibits isoform F2 and slightly isoform F1 Sus scrofa
SO42- inhibits only isoform F1 Sus scrofa
sorbinil inhibits isoform F1 Sus scrofa
Thiol-blocking reagents F1 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 35000, F2, SDS-PAGE Sus scrofa
39000
-
2 * 39000, F1, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
naphthalene dihydrodiol + NAD(P)+ Sus scrofa
-
?
-
?
trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+ Sus scrofa
-
catechol + NAD(P)H
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
at least 2 proteins: monomeric form F2 and dimeric form F1 with both dihydrodiol dehydrogenase EC 1.3.1.20 and aldehyde reductase activity EC 1.1.1.2, F1 shows higher dihydrodiol activity, F2 shows higher aldehyde reductase activity
-

Purification (Commentary)

Purification (Comment) Organism
at least 2 proteins: monomeric form F2 and dimeric form F1 with both dihydrodiol dehydrogenase EC 1.3.1.20 and aldehyde reductase activity EC 1.1.1.2 Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ probably identical with aldehyde reductase EC 1.1.1.2 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland low activity Sus scrofa
-
brain low activity Sus scrofa
-
lens
-
Sus scrofa
-
lung low activity Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-glyceraldehyde + NADPH probable identity with aldehyde reductase EC 1.1.1.2 Sus scrofa ?
-
?
additional information at least 2 proteins, monomeric form F2 and dimeric form F1, with both dihydrodiol dehydrogenase EC 1.3.1.20 and aldehyde reductase activity EC 1.1.1.2, F1: specifically oxidizes trans-hydrodiols of naphthalene and benzene strictly with NADP+ as cofactor, reduction of vicinal diketones, aromatic aldehydes and glyceraldes with NADPH as cofactor Sus scrofa additional information
-
?
additional information not: hydroxy steroids Sus scrofa additional information
-
?
additional information F2: high activity towards naphthalene dihydrodiol and some alcohols, low activity towards trans- and cis-benzene dihydrodiols and some alcohols, reduction of aldoses, vicinal diketones, aromatic aldehydes and glyceraldehydes with NADPH or NADH as cofactors Sus scrofa additional information
-
?
naphthalene dihydrodiol + NAD(P)+
-
Sus scrofa ?
-
?
trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+
-
Sus scrofa catechol + NAD(P)H
-
?
trans-naphthalene dihydrodiol + NAD(P)+
-
Sus scrofa ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 39000, F1, SDS-PAGE Sus scrofa
monomer 1 * 35000, F2, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
F2: reduction of vicinal diketones, aromatic aldehydes and glyceraldehydes + NAD(P)H Sus scrofa
6 8 F1: reduction of vicinal diketones, aromatic aldehydes and glyceraldehydes + NADPH Sus scrofa
7.6
-
F2: oxidation of naphthalene dihydrodiol + NAD(P)+ Sus scrofa
10.2
-
F1: oxidation of trans-dihydrodiols of naphthalene and benzene + NADP+ Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sus scrofa
NADH activity only with isoform F2 Sus scrofa
NADP+
-
Sus scrofa
NADPH
-
Sus scrofa