Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants, H79Q and Y180F, in Escherichia coli | Macaca fuscata |
Protein Variants | Comment | Organism |
---|---|---|
H79Q | site-directed mutagenesis, moderate activity with 190fold increase in Km-value, inhibition by MgCl2 and potassium phosphate | Macaca fuscata |
additional information | sequence analysis reveals high sequence homology between dimeric mammalian dihydrodiol dehydrogenases, but no homology with monomeric or cis-dihydrodiol-specific enzymes | Macaca fuscata |
Y180F | site-directed mutagenesis, very low activity | Macaca fuscata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | inhibition of mutant enzymes H79Q and Y180F | Macaca fuscata | |
phosphate buffer | inhibitionn of mutant enzymes H79Q and Y180F | Macaca fuscata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Camphorquinone | NADPH | Macaca fuscata | |
0.51 | - |
Camphorquinone | mutant H79Q, NADPH | Macaca fuscata | |
0.55 | - |
NADP+ | phosphate buffer 0.1 M | Macaca fuscata | |
0.56 | - |
NADPH | - |
Macaca fuscata | |
2.1 | - |
NADP+ | Tris buffer 25 mM | Macaca fuscata | |
4.4 | - |
D-xylose | NADP+, 25 mM Tris buffer 25 mM | Macaca fuscata | |
6.3 | - |
D-xylose | NADP+, 0.1 M phosphate buffer | Macaca fuscata | |
14 | - |
NADP+ | mutant H79Q, phosphate buffer 0.1 M | Macaca fuscata | |
28 | - |
NADPH | mutant H79Q | Macaca fuscata | |
67 | - |
D-xylose | mutant H79Q, NADP+, 0.1 M phosphate buffer | Macaca fuscata | |
213 | - |
D-xylose | mutant H79Q, NADP+, Tris buffer 25 mM | Macaca fuscata | |
402 | - |
NADP+ | mutant H70Q, Tris buffer 25 mM | Macaca fuscata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Macaca fuscata |
70000 | - |
gel filtration | Macaca fuscata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macaca fuscata | - |
japanese monkey | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutant protein recombinant from Escherichia coli | Macaca fuscata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | identical with D-xylose dehydrogenase EC 1.1.1.179 | Macaca fuscata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Macaca fuscata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
camphorquinone + NAD(P)H | - |
Macaca fuscata | (1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+ | - |
? | |
D-xylose + NADP+ | - |
Macaca fuscata | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
additional information | monomeric forms are identical with 3alpha-hydroxysteroid dehydrogenase EC 1.1.1.50 and provide a broad substrate specificity, while dimeric forms are identical with D-xylose dehydrogenase EC 1.1.1.179 and are active with trans-dihydrodiols and dicarbonyl compounds | Macaca fuscata | additional information | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Macaca fuscata |
Synonyms | Comment | Organism |
---|---|---|
DD | - |
Macaca fuscata |
dihydrodiol dehydrogenase | - |
Macaca fuscata |
More | sequence analysis revealed high sequence homology between dimeric mammalian dihydrodiol dehydrogenases, but no homology with monomeric or cis-dihydrodiol-specific enzymes | Macaca fuscata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Macaca fuscata | |
NADPH | - |
Macaca fuscata |