KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
naphthalene-cis-1,2-dihydrodiol | pH 7.2, 45°C | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
6 * 27000, SDS-PAGE | Pseudomonas fluorescens |
160000 | - |
gel filtration | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
naphthalene-cis-1,2-dihydrodiol + NAD+ | Pseudomonas fluorescens | - |
? | - |
? | |
naphthalene-cis-1,2-dihydrodiol + NAD+ | Pseudomonas fluorescens 26K | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens 26K | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from strain 26K by anion exchange and hydrophobic interaction chromatography | Pseudomonas fluorescens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:naphthalene-grown cell | - |
Pseudomonas fluorescens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.098 | - |
crude enzyme extract, pH 7.2, 45°C | Pseudomonas fluorescens |
5.1 | - |
purified native enzyme, pH 7.2, 45°C | Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
enzyme activity is retained during purification and storage in 100 mM Tris-HCl buffer, pH 7.2, containing 5% ethanol | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NDDH is inactive against cis-1,2-dihydronaphthalene and salicylaldehyde | Pseudomonas fluorescens | ? | - |
? | |
additional information | NDDH is inactive against cis-1,2-dihydronaphthalene and salicylaldehyde | Pseudomonas fluorescens 26K | ? | - |
? | |
naphthalene-cis-1,2-dihydrodiol + NAD+ | - |
Pseudomonas fluorescens | ? | - |
? | |
naphthalene-cis-1,2-dihydrodiol + NAD+ | - |
Pseudomonas fluorescens 26K | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 27000, SDS-PAGE | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
naphthalene cis-1,2-dihydrodiol dehydrogenase | - |
Pseudomonas fluorescens |
NDDH | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas fluorescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 60 | activity range | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity range | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Pseudomonas fluorescens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the metabolism and degradation of naphthalene | Pseudomonas fluorescens |