General Stability | Organism |
---|---|
unstable | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
cis-1,2-dihydrobenzene-1,2-diol | cosubstrate NAD+ | Pseudomonas putida | |
0.22 | - |
NAD+ | - |
Pseudomonas putida | |
46 | - |
glycerol | cosubstrate NAD+ | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ions requirement | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
4 * 39000, SDS-PAGE | Pseudomonas putida |
166000 | - |
PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-1,2-dihydrobenzene-1,2-diol + NAD+ | Pseudomonas putida | - |
catechol + NADH | - |
? | |
cis-1,2-dihydrobenzene-1,2-diol + NAD+ | Pseudomonas putida ML2 | - |
catechol + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida ML2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
dye-affinity chromatography and NAD+ agarose chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
68 | - |
glycerol, NAD+ | Pseudomonas putida |
153 | - |
cis-1,2-dihydrobenzene-1,2-diol, NAD+ | Pseudomonas putida |
313 | - |
cis-1,2-cyclohexanediol, NAD+ | Pseudomonas putida |
513 | - |
1,2-propanediol, NAD+ | Pseudomonas putida |
Storage Stability | Organism |
---|---|
less stable at 4°C than at -20°C, loss of activity at 4°C gradually over a few days | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + NAD+ | - |
Pseudomonas putida | propen-1,2-diol + NADH | - |
? | |
1,2-propanediol + NAD+ | - |
Pseudomonas putida ML2 | propen-1,2-diol + NADH | - |
? | |
cis-1,2-cyclohexanediol + NAD+ | - |
Pseudomonas putida | cyclohex-1-ene-1,2-diol + NADH | - |
? | |
cis-1,2-cyclohexanediol + NAD+ | - |
Pseudomonas putida ML2 | cyclohex-1-ene-1,2-diol + NADH | - |
? | |
cis-1,2-dihydrobenzene-1,2-diol + NAD+ | - |
Pseudomonas putida | catechol + NADH | - |
? | |
cis-1,2-dihydrobenzene-1,2-diol + NAD+ | - |
Pseudomonas putida ML2 | catechol + NADH | - |
? | |
glycerol + NAD+ | - |
Pseudomonas putida | ? | - |
? | |
glycerol + NAD+ | - |
Pseudomonas putida ML2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 39000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
cis-benzene dihydrodiol dehydrogenase | bedD enzyme | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme assay | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
with NAD+ as cofactor, 100 mM potassium carbonate buffer | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida |