Cloned (Comment) | Organism |
---|---|
for co-overexpression with disulfide isomerase in Pichia pastoris cells, for cloning of wild-type BBE, the mutant H104A and mutant H104A-C166A the vector pPICZalpha is used | Eschscholzia californica |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of the mutants H104A, C166A, C166A in complex with (S)-reticuline and of the wild-type enzyme in complex with (S)-scoulerine are determined | Eschscholzia californica |
Protein Variants | Comment | Organism |
---|---|---|
C166A | mutant, lacking one of the covalent linkages to the cofactor FAD | Eschscholzia californica |
H104A | mutant, lacking one of the covalent linkages to the cofactor FAD | Eschscholzia californica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-reticuline + O2 | Eschscholzia californica | - |
(S)-scoulerine + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eschscholzia californica | P30986 | - |
- |
Purification (Comment) | Organism |
---|---|
the secreted enzymes are purified by gel filtration and anion exchange chromatography | Eschscholzia californica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-reticuline + O2 | - |
Eschscholzia californica | (S)-scoulerine + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BBE | - |
Eschscholzia californica |
berberine bridge enzyme | - |
Eschscholzia californica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
(S)-reticuline | mutant C166A | Eschscholzia californica | |
0.54 | - |
(S)-reticuline | mutant H104A | Eschscholzia californica | |
8 | - |
(S)-reticuline | wild-type | Eschscholzia californica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Eschscholzia californica |