Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59599 | - |
x * 59599, calculated, including FAD cofactor | Eschscholzia californica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eschscholzia californica | - |
expression in Pichia pastoris | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | three potential N-glycosylation sites, one at the N-terminus, N38, and two at the C-terminus, Asn-423 and Asn-471 | Eschscholzia californica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | recombinant enzyme | Eschscholzia californica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-reticuline + O2 | - |
Eschscholzia californica | (S)-scoulerine + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59599, calculated, including FAD cofactor | Eschscholzia californica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.2 | - |
(S)-reticuline | pH 9.0, 37°C | Eschscholzia californica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently attached to enzyme at 8alpha position to H104 and also at 6 position to thiol group of C166. anaerobic photoirradiation leads to cleavage of the linkage to C166 yielding 6-mercaptoflavin and a peptide with C replaced by A due to breakage of the C-S bond | Eschscholzia californica |