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Literature summary for 1.2.7.3 extracted from

  • Yoon, K.S.; Ishii, M.; Igarashi, Y.; Kodama, T.
    Purification and characterization of 2-oxoglutarate:ferredoxin oxidoreductase from a thermophilic, obligately chemolithoautotrophic bacterium, Hydrogenobacter thermophilus TK-6 (1996), J. Bacteriol., 178, 3365-3368.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
Triton X-100 highly stablizes during purification Hydrogenobacter thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
CoA pH 7.6, 70°C Hydrogenobacter thermophilus
1.42
-
2-oxoglutarate pH 7.6, 70°C Hydrogenobacter thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Hydrogenobacter thermophilus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 70000 + 1 * 35000, alphabeta, SDS-PAGE Hydrogenobacter thermophilus
70000
-
1 * 70000 + 1 * 35000, alphabeta, SDS-PAGE Hydrogenobacter thermophilus
105000
-
gel filtration Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + CO2 + reduced ferredoxin Hydrogenobacter thermophilus involved in reductive TCA-cycle 2-oxoglutarate + CoA + oxidized ferredoxin
-
r
succinyl-CoA + CO2 + reduced ferredoxin Hydrogenobacter thermophilus TK-6 / IAM 12695 involved in reductive TCA-cycle 2-oxoglutarate + CoA + oxidized ferredoxin
-
r

Organism

Organism UniProt Comment Textmining
Hydrogenobacter thermophilus
-
-
-
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
pH 7.6, 70°C Hydrogenobacter thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + CoA + oxidized ferredoxin no activity with oxalacetate, oxomalonate, 2-oxoisocaproate, or phosphoenolpyruvate. The enzyme reacts with 2-oxobutyrate, pyruvate, and 2-oxoisovalerate at an activity level of less than 0.4 to 0.7% relative to that with 2-oxoglutarate. The enzyme uses 2-oxoglutarate, coenzyme A, and one of several electron acceptors in substrate amounts (ferredoxin isolated from Hydrogenobacter thermophilus, flavin adenine dinucleotide, flavin mononucleotide, or methyl viologen). NAD+, NADP+, and ferredoxins from Chlorella spp. and Clostridium pasteurianum are ineffective Hydrogenobacter thermophilus succinyl-CoA + CO2 + reduced ferredoxin
-
r
2-oxoglutarate + CoA + oxidized ferredoxin no activity with oxalacetate, oxomalonate, 2-oxoisocaproate, or phosphoenolpyruvate. The enzyme reacts with 2-oxobutyrate, pyruvate, and 2-oxoisovalerate at an activity level of less than 0.4 to 0.7% relative to that with 2-oxoglutarate. The enzyme uses 2-oxoglutarate, coenzyme A, and one of several electron acceptors in substrate amounts (ferredoxin isolated from Hydrogenobacter thermophilus, flavin adenine dinucleotide, flavin mononucleotide, or methyl viologen). NAD+, NADP+, and ferredoxins from Chlorella spp. and Clostridium pasteurianum are ineffective Hydrogenobacter thermophilus TK-6 / IAM 12695 succinyl-CoA + CO2 + reduced ferredoxin
-
r
succinyl-CoA + CO2 + reduced ferredoxin
-
Hydrogenobacter thermophilus 2-oxoglutarate + CoA + oxidized ferredoxin
-
r
succinyl-CoA + CO2 + reduced ferredoxin involved in reductive TCA-cycle Hydrogenobacter thermophilus 2-oxoglutarate + CoA + oxidized ferredoxin
-
r
succinyl-CoA + CO2 + reduced ferredoxin
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 2-oxoglutarate + CoA + oxidized ferredoxin
-
r
succinyl-CoA + CO2 + reduced ferredoxin involved in reductive TCA-cycle Hydrogenobacter thermophilus TK-6 / IAM 12695 2-oxoglutarate + CoA + oxidized ferredoxin
-
r

Subunits

Subunits Comment Organism
dimer 1 * 70000 + 1 * 35000, alphabeta, SDS-PAGE Hydrogenobacter thermophilus

Synonyms

Synonyms Comment Organism
2-oxoglutarate:ferredoxin oxidoreductase
-
Hydrogenobacter thermophilus
OGOR
-
Hydrogenobacter thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 85
-
Hydrogenobacter thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
22 h, anaerobic conditions, 50% loss of activity Hydrogenobacter thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 7.8 2-oxoglutarate oxidation reaction Hydrogenobacter thermophilus