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Literature summary for 1.2.3.3 extracted from

  • Sedewitz, B.; Schleifer, K.H.; Götz, F.
    Purification and biochemical characterization of pyruvate oxidase from Lactobacillus plantarum (1984), J. Bacteriol., 160, 273-278.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
EDTA 11% inhibition at 10 mM Lactiplantibacillus plantarum
KCN 25% inhibition at 10 mM Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
pyruvate electron acceptor: oxygen Lactiplantibacillus plantarum
1.25
-
arsenate electron acceptor: oxygen Lactiplantibacillus plantarum
2.3
-
phosphate electron acceptor: oxygen Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ most effective Lactiplantibacillus plantarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
4 * 68000, SDS-PAGE Lactiplantibacillus plantarum
262000
-
gel filtration Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + phosphate + O2 + H2O Lactiplantibacillus plantarum
-
acetyl phosphate + CO2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, ultracentrifugation and chromatography techniques Lactiplantibacillus plantarum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Lactiplantibacillus plantarum
-

Storage Stability

Storage Stability Organism
4°C, pH 5.7, several weeks Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + phosphate + O2 + H2O 15% of the activity compared to pyruvate as substrate Lactiplantibacillus plantarum ? + H2O2
-
?
alpha-ketobutyrate + phosphate + O2 + H2O very low activity Lactiplantibacillus plantarum ? + H2O2
-
?
methylglyoxal + phosphate + O2 + H2O 20% of the activity compared to pyruvate as substrate Lactiplantibacillus plantarum ? + H2O2
-
?
pyruvate + arsenate + O2 + H2O can replace phosphate, 25% higher activity compared to phosphate as cosubstrate Lactiplantibacillus plantarum acetyl arsenate + CO2 + H2O2
-
?
pyruvate + phosphate + 2,6-dichlorophenolindophenol + H2O 36% of the activity compared to oxygen as electron acceptor Lactiplantibacillus plantarum acetyl phosphate + CO2 + reduced 2,6-dichlorophenolindophenol + H2O2
-
?
pyruvate + phosphate + ferricyanide + H2O 65% of the activity compared to oxygen as electron acceptor Lactiplantibacillus plantarum acetyl phosphate + ferrocyanide + H2O2
-
?
pyruvate + phosphate + O2 + H2O
-
Lactiplantibacillus plantarum acetyl phosphate + CO2 + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 68000, SDS-PAGE Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
Lactiplantibacillus plantarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
unstable above Lactiplantibacillus plantarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.6 5.8 highest stability at 4°C and highest remaining activity of 67% Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
FAD tightly bound Lactiplantibacillus plantarum
thiamine diphosphate tightly bound Lactiplantibacillus plantarum