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Literature summary for 1.2.3.1 extracted from

  • Kuecuekgoeze, G.; Terao, M.; Garattini, E.; Leimkuehler, S.
    Direct comparison of the enzymatic characteristics and superoxide production of the four aldehyde oxidase enzymes present in mouse (2017), Drug Metab. Dispos., 45, 947-955 .
    View publication on PubMed
Search for more literature for 1.2.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escheirchia coli Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
0.0005
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
0.0008
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
0.0023
-
 Vanillin pH 8, 37°C Mus musculus
0.0033
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
0.0035
-
 Salicylaldehyde pH 8, 37°C Mus musculus
0.0046
-
 Vanillin pH 8, 37°C Mus musculus
0.0054
-
 Vanillin pH 8, 37°C Mus musculus
0.0094
-
 N1-methylnicotinamide pH 8, 37°C Mus musculus
0.0126
-
 retinalaldehyde pH 8, 37°C Mus musculus
0.0132
-
 Salicylaldehyde pH 8, 37°C Mus musculus
0.0165
-
 retinalaldehyde pH 8, 37°C Mus musculus
0.0211
-
 Salicylaldehyde pH 8, 37°C Mus musculus
0.0264
-
 retinalaldehyde pH 8, 37°C Mus musculus
0.0279
-
 N1-methylnicotinamide pH 8, 37°C Mus musculus
0.0321
-
 retinalaldehyde pH 8, 37°C Mus musculus
0.0896
-
 Salicylaldehyde pH 8, 37°C Mus musculus
0.104
-
 Vanillin pH 8, 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus G3X982 isoform Aox3
-
Mus musculus O54754 isoform AOX1
-
Mus musculus Q3TYQ9 isoform AOX4
-
Mus musculus Q5SGK3 isoform AOX2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, simplified two-step purification method using Ni-NTA and size-exclusion chromatography Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.146
-
 substrate salicylaldehyde, pH 8, 37°C, after chemical sulfuration of the molybdopterin cofactor Mus musculus
0.158
-
 substrate salicylaldehyde, pH 8, 37°C, after chemical sulfuration of the molybdopterin cofactor Mus musculus
0.585
-
 substrate salicylaldehyde, pH 8, 37°C, after chemical sulfuration of the molybdopterin cofactor Mus musculus
0.88
-
 substrate salicylaldehyde, pH 8, 37°C, after chemical sulfuration of the molybdopterin cofactor Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(dimethylamino)cinnamaldehyde + H2O + O2
-
 Mus musculus 4-(dimethylamino)cinnamic acid + H2O2
-
 ?
additional information no substrate for isoform AOX2: pyridoxal Mus musculus ?
-
-
additional information no substrate for isoform AOX3: pyridoxal Mus musculus ?
-
-
additional information no substrates for isoform AOX1: N1-methylnicotinamide, pyridoxal Mus musculus ?
-
-
additional information no substrates for isoform AOX4: N1-methylnicotinamide, pyridoxal Mus musculus ?
-
-
N1-methylnicotinamide + H2O + O2
-
 Mus musculus N1-methyl-2-pyridone-5-carboxamide + N1-methyl-4-pyridone-3-carboxamide + H2O2
-
 ?
retinalaldehyde + O2 + H2O
-
 Mus musculus retinoic acid + H2O2
-
 ?
salicylaldehyde + H2O + O2
-
 Mus musculus salicylic acid + H2O2
-
 ?
vanillin + H2O + O2
-
 Mus musculus vanillic acid + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 150000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
AOX1
-
 Mus musculus
AOX2
-
 Mus musculus
AOX3
-
 Mus musculus
AOX4
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 retinalaldehyde pH 8, 37°C Mus musculus
0.26
-
 Vanillin pH 8, 37°C Mus musculus
0.33
-
 N1-methylnicotinamide pH 8, 37°C Mus musculus
0.47
-
 retinalaldehyde pH 8, 37°C Mus musculus
0.51
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
0.75
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
0.82
-
 N1-methylnicotinamide pH 8, 37°C Mus musculus
1.34
-
 Vanillin pH 8, 37°C Mus musculus
1.47
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
1.94
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
2.05
-
 retinalaldehyde pH 8, 37°C Mus musculus
2.29
-
 Salicylaldehyde pH 8, 37°C Mus musculus
2.66
-
 Vanillin pH 8, 37°C Mus musculus
3.42
-
 Salicylaldehyde pH 8, 37°C Mus musculus
3.55
-
 Salicylaldehyde pH 8, 37°C Mus musculus
3.7
-
 Salicylaldehyde pH 8, 37°C Mus musculus
7.5
-
 Vanillin pH 8, 37°C Mus musculus

Cofactor

Cofactor Comment Organism Structure
molybdenum cofactor purified enzyme displays molybdenum saturation levels around 40% Mus musculus
molybdenum cofactor purified enzyme displays molybdenum saturation levels around 50% Mus musculus
[2Fe-2S]-center purified enzyme displays iron saturation levels around 60% Mus musculus

General Information

General Information Comment Organism
physiological function AOX2 is the isoform generating the largest rate of superoxide radicals of around 40% in relation to moles of substrate converted Mus musculus
physiological function isoform AOX1 is the homolog to the human enzyme and produces a rate of approximately 30% of superoxide radicals in relation to moles of substrate converted Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.7
-
 N1-methylnicotinamide pH 8, 37°C Mus musculus
13.3
-
 retinalaldehyde pH 8, 37°C Mus musculus
18.3
-
 retinalaldehyde pH 8, 37°C Mus musculus
25
-
 Salicylaldehyde pH 8, 37°C Mus musculus
48.3
-
 Vanillin pH 8, 37°C Mus musculus
71.7
-
 Vanillin pH 8, 37°C Mus musculus
87.7
-
 N1-methylnicotinamide pH 8, 37°C Mus musculus
123.3
-
 retinalaldehyde pH 8, 37°C Mus musculus
175
-
 Salicylaldehyde pH 8, 37°C Mus musculus
260
-
 Salicylaldehyde pH 8, 37°C Mus musculus
568.3
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
575
-
 Vanillin pH 8, 37°C Mus musculus
605
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
701.7
-
 Vanillin pH 8, 37°C Mus musculus
1006.7
-
 Salicylaldehyde pH 8, 37°C Mus musculus
2088.3
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus
3278.3
-
 4-(dimethylamino)cinnamaldehyde pH 8, 37°C Mus musculus