Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
phenylacetaldehyde | with NAD+ as cosubstrate, at pH, temperature not specified in the publication | Escherichia coli | |
0.5 | - |
NAD+ | with phenylacetaldehyde as cosubstrate, at pH 7.7, temperature not specified in the publication | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetaldehyde + NAD+ + H2O | Escherichia coli | - |
phenylacetate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P80668 | - |
- |
Storage Stability | Organism |
---|---|
-20°C, in 250 mM Tris-HCl pH 7.0, 3 months, 52% loss of activity | Escherichia coli |
0°C, in 250 mM Tris-HCl pH 7.0, 3 months, 46% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chlorophenylacetaldehyde + NAD+ + H2O | high activity | Escherichia coli | 4-chlorophenylacetate + NADH + 2 H+ | - |
? | |
4-fluorophenylacetaldehyde + NAD+ + H2O | high activity | Escherichia coli | 4-fluorophenylacetate + NADH + 2 H+ | - |
? | |
additional information | no activity with phenazine methosulfate | Escherichia coli | ? | - |
- |
|
phenylacetaldehyde + NAD+ + H2O | - |
Escherichia coli | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | highest activity | Escherichia coli | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NADP+ + H2O | 11% activity compared to NAD+ | Escherichia coli | phenylacetate + NADPH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
feaB | - |
Escherichia coli |
NAD(P)+-dependent phenylacetaldehyde dehydrogenase | - |
Escherichia coli |
PAD | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
55 | after preincubation of the protein for 30 min at 0 to 45°C, residual activities of 82.7 to 100% are measured. After the incubation for 30 min at 50°C, this enzyme shows an activity of about 63.1%, whereas significant enzyme inactivation is observed after incubation at 55°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the most suitable co-substrate is NAD+ | Escherichia coli |