Cloned (Comment) | Organism |
---|---|
gene ebA4954, recombinant expression of N-terminally Strep-tagged enzyme in Escherichia coli strain DHalpha | Aromatoleum aromaticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | at high concentrations | Aromatoleum aromaticum | |
NAD+ | cooperative substrate inhibition | Aromatoleum aromaticum | |
NADP+ | cooperative substrate inhibition | Aromatoleum aromaticum | |
phenylacetaldehyde | the enzyme is highly specific for phenylacetaldehyde, has cooperative kinetics toward the substrate, and shows considerable substrate inhibition | Aromatoleum aromaticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis, overview. The enzyme shows cooperative kinetics toward substrate phenylacetaldehyde and considerable substrate inhibition | Aromatoleum aromaticum | |
0.018 | - |
NAD+ | pH 8.5, 28°C | Aromatoleum aromaticum | |
0.025 | - |
NADP+ | pH 8.5, 28°C | Aromatoleum aromaticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetaldehyde + NAD+ + H2O | Aromatoleum aromaticum | - |
phenylacetate + NADH + 2 H+ | - |
r | |
phenylacetaldehyde + NAD+ + H2O | Aromatoleum aromaticum EbN1 | - |
phenylacetate + NADH + 2 H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aromatoleum aromaticum | Q5P171 | - |
- |
Aromatoleum aromaticum EbN1 | Q5P171 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 126fold by cross-flow filtration, ultracentrifugation, and anion exchange chromatography, recombinant Strep-tagged enzyme 137fold from Escherichia coli strain DHalpha by affinity chromatography on a Strep-Tactin column | Aromatoleum aromaticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.102 | - |
purified native enzyme, with cofactor NADP+, pH 8.5, 28°C | Aromatoleum aromaticum |
0.21 | - |
purified recombinant Strep-tagged enzyme, pH 8.5, 28°C | Aromatoleum aromaticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is highly specific for phenylacetaldehyde, has cooperative kinetics toward the substrate, and shows considerable substrate inhibition. No activity with benzaldehyde and indole-3-carbaldehyde, substrate specificity, overview | Aromatoleum aromaticum | ? | - |
? | |
additional information | enzyme is highly specific for phenylacetaldehyde, has cooperative kinetics toward the substrate, and shows considerable substrate inhibition. No activity with benzaldehyde and indole-3-carbaldehyde, substrate specificity, overview | Aromatoleum aromaticum EbN1 | ? | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Aromatoleum aromaticum | phenylacetate + NADH + 2 H+ | - |
r | |
phenylacetaldehyde + NAD+ + H2O | - |
Aromatoleum aromaticum EbN1 | phenylacetate + NADH + 2 H+ | - |
r | |
phenylacetaldehyde + NADP+ + H2O | - |
Aromatoleum aromaticum | phenylacetate + NADPH + 2 H+ | - |
r | |
phenylacetaldehyde + NADP+ + H2O | - |
Aromatoleum aromaticum EbN1 | phenylacetate + NADPH + 2 H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 53500, recombinant Strep-tagged enzyme, SDS-PAGE | Aromatoleum aromaticum |
Synonyms | Comment | Organism |
---|---|---|
ebA4954 | - |
Aromatoleum aromaticum |
PDH | - |
Aromatoleum aromaticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Aromatoleum aromaticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
53 | - |
NAD+ | pH 8.5, 28°C | Aromatoleum aromaticum | |
63 | - |
NADP+ | pH 8.5, 28°C | Aromatoleum aromaticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | assay at, reverse reaction | Aromatoleum aromaticum |
8.5 | - |
assay at, forward reaction | Aromatoleum aromaticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme PDH accepts both NAD+ and NADP+ as electron acceptors, but exhibits 86% of its maximal activity with NAD+ but only 5% of its maximal activity with NADP+ | Aromatoleum aromaticum | |
NAD+ | - |
Aromatoleum aromaticum | |
NADH | - |
Aromatoleum aromaticum | |
NADP+ | - |
Aromatoleum aromaticum | |
NADPH | - |
Aromatoleum aromaticum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
NADP+ | pH 8.5, 28°C, cooperative substrate inhibition | Aromatoleum aromaticum | |
0.069 | - |
NAD+ | pH 8.5, 28°C, cooperative substrate inhibition | Aromatoleum aromaticum |
General Information | Comment | Organism |
---|---|---|
metabolism | two distinct enzymes are involved in the oxidation of phenylacetaldehyde to phenylacetate, an aldehyde:ferredoxin oxidoreductase (AOR) and a phenylacetaldehyde dehydrogenase (PDH). PDH is the primary enzyme during anaerobic phenylalanine degradation, whereas AOR is not essential for the metabolic pathway and exhibits probably a function as a detoxifying enzyme if high aldehyde concentrations accumulate in the cytoplasm, which would lead to substrate inhibition of PDH | Aromatoleum aromaticum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
NADP+ | pH 8.5, 28°C | Aromatoleum aromaticum | |
0.003 | - |
NAD+ | pH 8.5, 28°C | Aromatoleum aromaticum |