Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-Quinolinol | 34% inhibition in the presence of 1 mM | Brevibacterium sp. | |
Ba2+ | 19% inhibition in the presence of 1 mM | Brevibacterium sp. | |
Cu2+ | 44% inhibition in the presence of 1 mM | Brevibacterium sp. | |
EDTA | 18% inhibition in the presence of 1 mM | Brevibacterium sp. | |
Hg2+ | 77% inhibition in the presence of 1 mM | Brevibacterium sp. | |
hydrozine | 51% inhibition in the presence of 1 mM | Brevibacterium sp. | |
iodoacetamide | completely inhibits in the presence of 1 mM | Brevibacterium sp. | |
iodoacetate | 18% inhibition in the presence of 1 mM | Brevibacterium sp. | |
N-ethylmaleinimide | completely inhibits in the presence of 1 mM | Brevibacterium sp. | |
NaN3 | 25% inhibition in the presence of 1 mM | Brevibacterium sp. | |
Ni2+ | 12% inhibition in the presence of 1 mM | Brevibacterium sp. | |
p-chloromercuribenzoate | completely inhibits in the presence of 1 mM | Brevibacterium sp. | |
phenylacetaldehyde | high concentration (0.1 mM) inhibits | Brevibacterium sp. | |
Zn2+ | 41% inhibition in the presence of 1 mM | Brevibacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00124 | - |
phenylacetaldehyde | - |
Brevibacterium sp. | |
0.116 | - |
NAD+ | - |
Brevibacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Brevibacterium sp. | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM stimulates by 53% | Brevibacterium sp. | |
Mg2+ | 1 mM stimulates by 42% | Brevibacterium sp. | |
Mn2+ | 1 mM stimulates by 17% | Brevibacterium sp. | |
additional information | is not stimulated by 10 mM KCl | Brevibacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
4 * 61000, SDS-PAGE | Brevibacterium sp. |
219000 | - |
gel filtration | Brevibacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetaldehyde + NAD+ + H2O | Brevibacterium sp. | - |
phenylacetate + NADH + H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | Brevibacterium sp. KU1309 | - |
phenylacetate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sp. | - |
- |
- |
Brevibacterium sp. KU1309 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, 15.9fold with a 0.21% yield | Brevibacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.261 | - |
cell-free extract | Brevibacterium sp. |
4.16 | - |
15.9fold purified enzyme | Brevibacterium sp. |
Storage Stability | Organism |
---|---|
4°C, 10 mM Tris-HCl buffer | Brevibacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthaldehyde + NAD+ + H2O | 24% activity relative to phenylacetaldehyde | Brevibacterium sp. | 1-naphthoate + NADH + H+ | - |
? | |
3-phenylpropionaldehyde + NAD+ + H2O | 34% activity relative to phenylacetaldehyde | Brevibacterium sp. | 3-phenylpropionate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 54% activity relative to phenylacetaldehyde | Brevibacterium sp. | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 54% activity relative to phenylacetaldehyde | Brevibacterium sp. KU1309 | benzoate + NADH + H+ | - |
? | |
decanal + NAD+ + H2O | 17% activity relative to phenylacetaldehyde | Brevibacterium sp. | decanoate + NADH + H+ | - |
? | |
hexanal + NAD+ + H2O | 21% activity relative to phenylacetaldehyde | Brevibacterium sp. | hexanoate + NADH + H+ | - |
? | |
hexanal + NAD+ + H2O | 21% activity relative to phenylacetaldehyde | Brevibacterium sp. KU1309 | hexanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | 31% activity relative to phenylacetaldehyde | Brevibacterium sp. | octanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | 31% activity relative to phenylacetaldehyde | Brevibacterium sp. KU1309 | octanoate + NADH + H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Brevibacterium sp. | phenylacetate + NADH + H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | best substrate | Brevibacterium sp. | phenylacetate + NADH + H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Brevibacterium sp. KU1309 | phenylacetate + NADH + H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | best substrate | Brevibacterium sp. KU1309 | phenylacetate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 61000, SDS-PAGE | Brevibacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
PADH | - |
Brevibacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Brevibacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Brevibacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Brevibacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | at pH 8.0, PADH retains 60% of the initial activity | Brevibacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Brevibacterium sp. |