Cloned (Comment) | Organism |
---|---|
- |
Pisum sativum |
Crystallization (Comment) | Organism |
---|---|
isozyme AMADH1 in complex with NAD+, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 13% (w/v) PEG 6000 and 5% (v/v) 2-methyl-2,4-pentanediol, at 20°C | Pisum sativum |
isozyme AMADH2 in complex with NAD+, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 18% (w/v) PEG 4000, 10% (v/v) isopropanol and 0.5% (w/v) n-octyl beta-D-glucopyranoside, at 20°C | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
4-guanidinobutyraldehyde | isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum | |
0.01 | - |
N,N,N-trimethyl-4-aminobutyraldehyde | isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum | |
0.01 | - |
3-aminopropionaldehyde | isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum | |
0.011 | - |
4-guanidinobutyraldehyde | isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum | |
0.021 | - |
N,N,N-trimethyl-4-aminobutyraldehyde | isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum | |
0.029 | - |
4-Aminobutyraldehyde | isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum | |
0.04 | - |
NAD+ | isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum | |
0.055 | - |
NAD+ | isozyme AMADH2, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum | |
0.075 | - |
3-aminopropionaldehyde | isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum | |
0.17 | - |
4-Aminobutyraldehyde | isozyme AMADH1, in 0.15 M Tris-HCl buffer (pH 9.0), at 20°C | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
117000 | - |
gel filtration | Pisum sativum |
120000 | - |
gel filtration | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | Q8VWZ1 | isozyme AMADH1 | - |
Pisum sativum | Q93YB2 | isozyme AMADH2 | - |
Purification (Comment) | Organism |
---|---|
cobalt- or nickel-charged IDA-Sepharose column chromatography and Resource Q column chromatography | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminopropionaldehyde + NAD+ + H2O | 100% activity | Pisum sativum | 3-aminopropanoate + NADH + H+ | - |
? | |
3-cyanopropionaldehyde + NAD+ + H2O | 108% activity compared to 3-aminopropionaldehyde | Pisum sativum | 3-cyanopropanoate + NADH + H+ | - |
? | |
3-cyanopropionaldehyde + NAD+ + H2O | 99% activity compared to 3-aminopropionaldehyde | Pisum sativum | 3-cyanopropanoate + NADH + H+ | - |
? | |
3-guanidinopropionaldehyde + NAD+ + H2O | 12% activity compared to 3-aminopropionaldehyde | Pisum sativum | 3-guanidinopropanoate + NADH + H+ | - |
? | |
3-guanidinopropionaldehyde + NAD+ + H2O | 17% activity compared to 3-aminopropionaldehyde | Pisum sativum | 3-guanidinopropanoate + NADH + H+ | - |
? | |
4-amino-2-hydroxybutyraldehyde + NAD+ + H2O | 13% activity compared to 3-aminopropionaldehyde | Pisum sativum | 4-amino-2-hydroxybutanoate + NADH + H+ | - |
? | |
4-amino-2-hydroxybutyraldehyde + NAD+ + H2O | 20% activity compared to 3-aminopropionaldehyde | Pisum sativum | 4-amino-2-hydroxybutanoate + NADH + H+ | - |
? | |
4-aminobutyraldehyde + NAD+ + H2O | 36% activity compared to 3-aminopropionaldehyde | Pisum sativum | 4-aminobutanoate + NADH + H+ | - |
? | |
4-aminobutyraldehyde + NAD+ + H2O | 82% activity compared to 3-aminopropionaldehyde | Pisum sativum | 4-aminobutanoate + NADH + H+ | - |
? | |
4-guanidino-2-hydroxybutyraldehyde + NAD+ + H2O | 22% activity compared to 3-aminopropionaldehyde | Pisum sativum | 4-guanidino-2-hydroxybutanoate + NADH + H+ | - |
? | |
4-guanidino-2-hydroxybutyraldehyde + NAD+ + H2O | 25% activity compared to 3-aminopropionaldehyde | Pisum sativum | 4-guanidino-2-hydroxybutanoate + NADH + H+ | - |
? | |
4-guanidinobutyraldehyde + NAD+ + H2O | 29% activity compared to 3-aminopropionaldehyde | Pisum sativum | 4-guanidinobutanoate + NADH + H+ | - |
? | |
4-guanidinobutyraldehyde + NAD+ + H2O | 30% activity compared to 3-aminopropionaldehyde | Pisum sativum | 4-guanidinobutanoate + NADH + H+ | - |
? | |
additional information | 3-acetylpyridine-NAD+ is the best electron acceptor and leads to 33% activity compared to that with NAD+, while thio-NAD+ drastically and especially affects isozyme AMADH1 activity. 3-Pyridinealdehyde-NAD+ hardly functions as a coenzyme for AMADH1. Deamino-NAD+ is a better coenzyme than NAD+ and increases the reaction rate by 52% | Pisum sativum | ? | - |
? | |
additional information | 3-acetylpyridine-NAD+ is the best electron acceptor and leads to 33% activity compared to that with NAD+, while thio-NAD+ drastically and especially affects isozyme AMADH1 activity. 3-Pyridinealdehyde-NAD+ hardly functions as a coenzyme for AMADH1. Deamino-NAD+ is a better coenzyme than NAD+ and increases the reaction rate by 72% | Pisum sativum | ? | - |
? | |
additional information | isozyme AMADH1 preferentially oxidizes C3 and C4 aminoaldehydes and has no activity with butyraldehyde, acetaldehyde, propionaldehyde, betaine aldehyde, valeraldehyde, capronaldehyde, enanthaldehyde, 2-pyridine carboxaldehyde, 3-pyridine carboxaldehyde, and 4-pyridine carboxaldehyde | Pisum sativum | ? | - |
? | |
additional information | isozyme AMADH2 preferentially oxidizes C3 and C4 aminoaldehydes and has no activity with butyraldehyde, acetaldehyde, propionaldehyde, betaine aldehyde, valeraldehyde, capronaldehyde, enanthaldehyde, 2-pyridine carboxaldehyde, 3-pyridine carboxaldehyde, and 4-pyridine carboxaldehyde | Pisum sativum | ? | - |
? | |
N,N,N-trimethyl-3-aminopropionaldehyde + NAD+ + H2O | 35% activity compared to 3-aminopropionaldehyde | Pisum sativum | gamma-butyrobetaine + NADH + H+ | - |
? | |
N,N,N-trimethyl-3-aminopropionaldehyde + NAD+ + H2O | 54% activity compared to 3-aminopropionaldehyde | Pisum sativum | gamma-butyrobetaine + NADH + H+ | - |
? | |
N,N,N-trimethyl-4-aminobutyraldehyde + NAD+ + H2O | 45% activity compared to 3-aminopropionaldehyde | Pisum sativum | N,N,N-trimethyl-4-aminobutanoate + NADH + H+ | - |
? | |
N,N,N-trimethyl-4-aminobutyraldehyde + NAD+ + H2O | 48% activity compared to 3-aminopropionaldehyde | Pisum sativum | N,N,N-trimethyl-4-aminobutanoate + NADH + H+ | - |
? | |
N,N-dimethyl-4-aminobutyraldehyde + NAD+ + H2O | 57% activity compared to 3-aminopropionaldehyde | Pisum sativum | N,N-dimethyl-4-aminobutanoate + NADH + H+ | - |
? | |
N,N-dimethyl-4-aminobutyraldehyde + NAD+ + H2O | 80% activity compared to 3-aminopropionaldehyde | Pisum sativum | N,N-dimethyl-4-aminobutanoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
4-aminobutyraldehyde dehydrogenase | - |
Pisum sativum |
ABALDH | - |
Pisum sativum |
AMADH1 | isozyme | Pisum sativum |
AMADH2 | isozyme | Pisum sativum |
aminoaldehyde dehydrogenase | - |
Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred cofactor over NADP+ | Pisum sativum | |
NADP+ | NADP+ is a poor electron acceptor compared to NAD+ reducing the activity of isozyme AMDH1 by 86% | Pisum sativum | |
NADP+ | NADP+ is a poor electron acceptor compared to NAD+ reducing the activity of isozyme AMDH2 by 85% | Pisum sativum |
General Information | Comment | Organism |
---|---|---|
metabolism | AMADH participates in carnitine biosynthesis in plants | Pisum sativum |