Application | Comment | Organism |
---|---|---|
synthesis | production of the functional pyruvate dehydrogenase complex. All components are coexpressed in the cytoplasm of baculovirus-infected SF9 cells by deletion of the mitochondrial localization signal sequences and E1a is FLAG-tagged to facilitate purification. The protein complex is purified using FLAG-M2 affinity resin, followed by Superdex 200 sizing chromatography. The E2 and E3BP components are then lipoylated in vitro. The resulting complex is over 90% pure and homogenous | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08559 and P11177 and O00330 and P10515 and P09622 | P08559 i.e. subunit PdhA1, cf. EC 1.2.4.1, P11177 i.e. subunit PdhB1, cf. EC 1.2.4.1, O00330 i.e. subunit PdhX, P10515 i.e. subunit DlaT, cf. EC 2.3.1.12, P09622 i.e. subunit Dld, cf. EC 1.8.1.4, respectively | - |
Synonyms | Comment | Organism |
---|---|---|
DLAT | - |
Homo sapiens |
DLD | - |
Homo sapiens |
PdhA1 | - |
Homo sapiens |
PdhB1 | - |
Homo sapiens |
PdhX | - |
Homo sapiens |