Protein Variants | Comment | Organism |
---|---|---|
Y177A | 11% residual pyruvate dehydrogenase multienzyme complex activity, binding of thiamine diphosphate is unaffected | Escherichia coli |
Y177F | 7% residual pyruvate dehydrogenase multienzyme complex activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
thiamin 2-thiazolone diphosphate | tight-binding inhibitor, binds via a two-step mechanism, | Escherichia coli | |
thiamin 2-thiothiazolone diphosphate | tight-binding inhibitor, binds via a two-step mechanism to wild-type, but to mutant Y177A via a one-step mechanism | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00158 | - |
thiamine diphosphate | wild-type, Hill coefficient 1.38, pH 7.0, 30°C | Escherichia coli | |
0.00665 | - |
thiamine diphosphate | mutant Y177A, Hill coefficient 1.0, pH 7.0, 30°C | Escherichia coli | |
0.28 | - |
pyruvate | mutant Y177A, pH 7.0, 30°C | Escherichia coli | |
0.515 | - |
pyruvate | wild-type, pH 7.0, 30°C | Escherichia coli | |
0.531 | - |
pyruvate | mutant Y177F, pH 7.0, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AFG8 | P0AFG8 i.e. pyruvate dehydrogenase E1 component, cf. EC 1.2.4.1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + dichlorophenol indophenol | - |
Escherichia coli | acetyl-CoA + CO2 + ? | - |
? | |
pyruvate + CoA + thiamine diphosphate | - |
Escherichia coli | acetyl-CoA + CO2 + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aceE | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.31 | - |
thiamine diphosphate | mutant Y177A, Hill coefficient 1.0, pH 7.0, 30°C | Escherichia coli | |
28.89 | - |
thiamine diphosphate | wild-type, Hill coefficient 1.38, pH 7.0, 30°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
pyruvate | mutant Y177A, pH 7.0, 30°C | Escherichia coli | |
56 | - |
pyruvate | wild-type, pH 7.0, 30°C | Escherichia coli | |
498 | - |
thiamine diphosphate | mutant Y177A, Hill coefficient 1.0, pH 7.0, 30°C | Escherichia coli | |
18000 | - |
thiamine diphosphate | wild-type, Hill coefficient 1.38, pH 7.0, 30°C | Escherichia coli |