Protein Variants | Comment | Organism |
---|---|---|
additional information | study on impact of N-terminal truncation on interaction with ferredoxin using deletions of up to 25 amino acids. Similar kcat values for cytochrome c reduction are measured for all but the most truncated pFNRII[N-5]DEGV which contains a truncated N-terminal domain beginning after the KISKK domain. The longer forms of isoform FNRII bind more strongly to a ferredoxin affinity column than the shorter forms. Mutants bearing lysine-to-glutamine mutations in the KISKK domain are affected in binding to the ferredoxin column | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00205 | - |
ferricytochrome c | mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
0.00312 | - |
ferricytochrome c | wild-type, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
0.0035 | - |
ferricytochrome c | mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
0.00999 | - |
ferricytochrome c | mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
0.0103 | - |
ferricytochrome c | mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
0.0104 | - |
ferricytochrome c | mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
0.01103 | - |
ferricytochrome c | mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication | Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Triticum aestivum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c2 + NADPH | - |
Triticum aestivum | 2 ferrocytochrome c2 + NADP+ + H+ | - |
? | |
ferricytochrome c + NADP+ | - |
Triticum aestivum | ferrocytochrome c + NADPH + H+ | - |
r | |
ferrocytochrome c + NADP+ | - |
Triticum aestivum | ferricytochrome c + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
FNRII | - |
Triticum aestivum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.1 | - |
reduced flavodoxin | mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
45.4 | - |
reduced flavodoxin | mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
65 | - |
reduced flavodoxin | mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
66.3 | - |
reduced flavodoxin | mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
104 | - |
reduced flavodoxin | wild-type, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
128.2 | - |
reduced flavodoxin | mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
139.4 | - |
reduced flavodoxin | mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication | Triticum aestivum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3700 | - |
ferricytochrome c | mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
4520 | - |
ferricytochrome c | mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
12640 | - |
ferricytochrome c | mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
12830 | - |
ferricytochrome c | mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
18940 | - |
ferricytochrome c | mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
31710 | - |
ferricytochrome c | mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication | Triticum aestivum | |
33330 | - |
ferricytochrome c | wild-type, pH 7.5, temperature not specified in the publication | Triticum aestivum |