Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dATP | the binding of effector dATP alters the active site to select for pyrimidines over purines. Crystal structures of Escherichia coli class Ia ribonucleotide reductase with all four substrate/specificity effector-pairs bound (CDP/dATP, UDP/dATP, ADP/dGTP, GDP/TTP) that reveal the conformational rearrangements responsible for this remarkable allostery. These structures delineate how ribonucleotide reductase reads the base of each effector and communicates substrate preference to the active site by forming differential hydrogen bonds, thereby maintaining the proper balance of deoxynucleotides in the cell | Escherichia coli | |
dGTP | the binding of effector dGTP alters the active site to select for ADP and GDP. Crystal structures of Escherichia coli class Ia ribonucleotide reductase with all four substrate/specificity effector-pairs bound (CDP/dATP, UDP/dATP, ADP/dGTP, GDP/TTP) that reveal the conformational rearrangements responsible for this remarkable allostery. These structures delineate how ribonucleotide reductase reads the base of each effector and communicates substrate preference to the active site by forming differential hydrogen bonds, thereby maintaining the proper balance of deoxynucleotides in the cell | Escherichia coli | |
TTP | the binding of effector TTP alters the active site to select for ADP and GDP. Crystal structures of Escherichia coli class Ia ribonucleotide reductase with all four substrate/specificity effector-pairs bound (CDP/dATP, UDP/dATP, ADP/dGTP, GDP/TTP) that reveal the conformational rearrangements responsible for this remarkable allostery. These structures delineate how ribonucleotide reductase reads the base of each effector and communicates substrate preference to the active site by forming differential hydrogen bonds, thereby maintaining the proper balance of deoxynucleotides in the cell | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystals are grown using the hanging drop vapor diffusion technique | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + thioredoxin | Escherichia coli | the enzyme converts ribonucleotides to deoxyribonucleotides, a reaction that is essential for DNA biosynthesis and repair | 2'-dADP + thioredoxin disulfide + H2O | - |
? | |
CDP + thioredoxin | Escherichia coli | the enzyme converts ribonucleotides to deoxyribonucleotides, a reaction that is essential for DNA biosynthesis and repair | 2'-dCDP + thioredoxin disulfide + H2O | - |
? | |
UDP + thioredoxin | Escherichia coli | the enzyme converts ribonucleotides to deoxyribonucleotides, a reaction that is essential for DNA biosynthesis and repair | 2'-dUDP + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00452 and P69924 | P00452: subunit NrdA, P69924: subunit NrdB | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + thioredoxin | - |
Escherichia coli | 2'-dADP + thioredoxin disulfide + H2O | - |
? | |
ADP + thioredoxin | the enzyme converts ribonucleotides to deoxyribonucleotides, a reaction that is essential for DNA biosynthesis and repair | Escherichia coli | 2'-dADP + thioredoxin disulfide + H2O | - |
? | |
CDP + thioredoxin | - |
Escherichia coli | 2'-dCDP + thioredoxin disulfide + H2O | - |
? | |
CDP + thioredoxin | the enzyme converts ribonucleotides to deoxyribonucleotides, a reaction that is essential for DNA biosynthesis and repair | Escherichia coli | 2'-dCDP + thioredoxin disulfide + H2O | - |
? | |
UDP + thioredoxin | - |
Escherichia coli | 2'-dUDP + thioredoxin disulfide + H2O | - |
? | |
UDP + thioredoxin | the enzyme converts ribonucleotides to deoxyribonucleotides, a reaction that is essential for DNA biosynthesis and repair | Escherichia coli | 2'-dUDP + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class Ia RNR | - |
Escherichia coli |
ribonucleotide reductase | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme converts ribonucleotides to deoxyribonucleotides, a reaction that is essential for DNA biosynthesis and repair | Escherichia coli |