Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 25 mM, maximal activation, 70% of activity with dithiothreitol | Oryctolagus cuniculus | |
dithioerythritol | 0.5 mM, maximal activation, 94% of activity with dithiothreitol | Oryctolagus cuniculus | |
dithiothreitol | optimal in vitro activity with 10 mM, inhibition above | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | higher than 10 mM, activation below | Oryctolagus cuniculus | |
Hydroxyurea | - |
Oryctolagus cuniculus | |
UDP | inhibition of CDP reduction | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
CDP | in the presence of 5'-adenylimidodiphosphate | Oryctolagus cuniculus | |
0.0023 | - |
CDP | in the presence of ATP | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, ATP-agarose, partial purification | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000045 | - |
partially purified enzyme, CDP reduction | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + reduced dithiothreitol | - |
Oryctolagus cuniculus | 2'-dCDP + oxidized dithiothreitol + H2O | - |
r | |
CDP + reduced dithiothreitol | - |
Oryctolagus cuniculus | dCDP + oxidized dithiothreitol + H2O | - |
r | |
ribonucleoside diphosphate + reduced thioredoxin | - |
Oryctolagus cuniculus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
UDP | - |
Oryctolagus cuniculus | |
0.69 | - |
Hydroxyurea | - |
Oryctolagus cuniculus |